Clarke, I.N.

Lambden, P.R.

Watson, M.W.

English

Research Repository

5300 Nucleic Acids Research, Vol. 18, No. 17The nucleotide sequence of the 6OkDa cysteine rich outermembrane protein of Chlamydia psittaci strain EAE/A22/MMark W.Watson, Paul R.Lambden and Ian N.ClarkeDepartment of Microbiology, University of Southampton Medical School, Southampton GeneralHospital, Southampton S09 4XY, UKSubmitted July 24, 1990 EMBL accession no. X53512Chlamydia are Gram negative bacteria which have an oligateintracellular life cycle. There are three species within the genusChlamydia; Chlamydia trachomatis, Chlamydia psittaci andChlamydia pneumoniae.C. psittaci shows a diverse host specificity causing latent andacute gastrointestinal infections in avian species, alsoconjunctivitis and pneumonitis in many feral and domesticatedmammals. Human infection by C. psittaci is rare.The chlamydial life cycle involves two morphological formsthe intracellular reticulate bodies (RBs) and the spore-likeelementary bodies (EBs). One major factor known to retardinfectious EB formation is cysteine deprivation (1). This maybe related to inhibition of the synthesis of the 60 kDa cysteinerich outer membrane protein (CrP). This protein is involved incross linking in the EB cell wall where it may serve to maintainthe structural integrity of the organism.The complete nucleotide sequence for the 60 kDa cysteine richouter membrane protein (CrP) of C. psittaci was determined usingdideoxy chain termination sequencing on double stranded plasmnidDNA. The sequence shows 70% and 71 % homology to theserovars LI and B 60 kDa CrP genes respectively (2). Thestructural gene consists of an open reading frame of 1671 basepairs starting with ATG at 190 bp and ending with a TAAtermination codon (Fig. 1).Structural analysis of the C. psittaci DNA sequence revealeda single hairpin loop 32 bp downstream of the termination codonwith a predicted free energy of -33 kcal mol-'. This hairpinloop was characteristic of a Rho-independent RNA-polymerasetranscription terminator with a run of four thymine residuesdownstream of the hairpin loop structure (3).ACKNOWLEDGEMENTSThis investigation received financial support from the specialprogramme of research, development and training in humanreproduction, World Health Organisation. M.W.W. is in receiptof an MRC research studentship.REFERENCES1. AllanI., Hatch,T.P. and Pearce,J.H. (1985) J. Gen. Microbiol. 131,3171 -3177.2. Watson,M.W., Lambden,P.R., Ward,M.E. and Clarke,I.N. (1989) FEMSMicrobiol. Lett. 65, 293-297.3. Rosenberg,M. and Court,D. (1979) Ann. Rev. Genet. 13, 319-353.1 CTAGACGGTAGATGCAAMCAATAGGTMACAGT TCTTATCTGT TTACT TAAAT TGGT TAGGCAGT TAGGAGGTAAAT TAT TTCCCTGC TAACT GCCT TTATGAAAAATAAAC TTA115 AATGTTGAGGGTAAGAGTTCACAACTTTCTACCCGATGGCAGAAGAAAAAATAACACACGCGATAGGAGATCCC TATGTCCAAACTCATCAGACGAGTAGTTACGGTCC TCGCGM S K L R R V V T V L A2 29 CTAACTAGTATGGCGAGTTCATTTGCCAGCGGGAAGATAGAGGCCGCTGCTGCAGAGTC TCTTGCTACAAGATTCATTGCCAGTAC TGAAAACGCAGATGACAATG TT T TTCAAL T S M A S S F A S G K E A A A A E S L A T R F I A S T E N A D D N V F Q343 GCAACAGCCAAGAAAGTTAGAT TTGGTCGTAACAAAAATCAAAGACAAGAACAAAAACATACTGAAGCT TTCTGTGATAAAGAATTTTATCC TTGTGAAGGTGGCCAGTGCCAAA T A K K V R F G R N K N Q R Q E Q K H T E A F C D K E F Y P C E G G Q C Q4 57 CCAGTAGACGCTACACAAGAATCTTGC TACGGCAAAATGTAT TGTGTCCGTGTTAACGATGACTGTAACGT TGAAATCAGCCAATC TGTACC TGAATATGCAACAGTAGGATC TP V D A T Q E S C Y G K M Y C V R V N D D C N V E S Q S V P E Y A T V G S571 CCTTATCCTATTGAAMTTCTCGCTGTAGGTAAAAAAGATTGCGTTAATGT TGTGAT TACTCAACAGCTTCCTTGCGAAGTTGAGT TTGTCAGCAGTGATCCTGCGACAACACCAP Y P I E I L A V G K K D C V N V V I T Q Q L P C E V E F V S S D P A T T P685 ACCTCGGATAGCAAATTAATCTGGACAATTGATCGCTTAGGTCAAGGTGAAAAATGCAAAMTTACCGTTTGGGTAAAACCTC TTAAAGAAGGT TGT TGCT TCACCGCGGC TAC TT S D S K L W T I D R L G Q G E K C K T V W V K P L K E G C C F T A A T799 GTATGTGCTTGCCCAGAAC TTCGC TCTTATACCMAAATGCGGACAACCAGC TAT T TGTATTAAGCAAGMAGGT CCTGAMTGCGCTTGC T TACGT TGCCCAGTTTGT TACAAAAT CV C A C P E L R S Y T K C G Q P A I C K Q E G P E C A C L R C P V C Y K I913 GAAGTTTGCAACACAGGTTCTGCTATAGCCCGTAATGTTGTCGTCGATAACCCAGTTCCCGATGGCTATACTCATGC TTCAGGACAACGCGT TCTTTCCTTTAACTTAGGAGATE V C N T G S A I A R N V V V D N P V P D G Y T H A S G Q R V 1. S F N L G D102 7 ATGCGTCC TGGGGAT TCTAAATGCTTCTGTGTGGAGTTTTGCCCACAAAAAGAGGAAAAG TTACTAACGTGGCTAC TGTATC TTACTGCGGAGGACATAAAT GTTCTGCTAACM R P G D S K C F C V E F C P Q K R G K V T N V A T V S Y C G G H K C S A N1141 GTAACTACTGTAGTTAACGAACCATGCGTACAAGTAAATATC TCTGGAGCTGAC TGGTCTTATGTATGTAMGCCTGTAGAATACACTATCGT TGTATC TAACCCAGGAGATCT TV T T V V N E P C V Q V N I S G A D W S Y V C K P V E Y T I V V S N P G D L12 55 AAMCTTTACGATGTAGTTATAGAAGATACCGCACC TTCAGGAGCAACAAT TTTAGAAGC TGCTGGAGCTGMAATCTGCTGTAACAAAGCTGTATGGTGCATCAAAGAAAT GTGCK L Y D V V I E D T A P S G A T I L E A A G A E I C C N K A V W C K E M C1369 CCAGGAGAGACTCTTCAATTTAAAGTTGTGGCTAAAGCACAAAGCCCAGGTAAATTCACAAATCAAGTTGTTGTCAAMACTAACTCCGATTGTGGAACCTGTACT TCT TGCGCAP G E T L Q F K V V A K A Q S P G K F T N Q V V V K T N S D C G T C T S C A1483 GAAGTTACAACACATTGGAAAGGTCTTGCAGCTACGCATATGTGCGTAATCGATACCAATGATCCTATTTGCGTAGGAGAAAATACTGTATACCGTAT TTGTGTAACCAACCGTE V T T H W K G L A A T H M C V I D T N D P I C V G E N T V Y R I C V T N R159 7 GGTTCTGCAGAAGATACGAACGTCTCGTTAATCCTTAAGTTT TCTAAGGAACTACAACCCGT TTCTTCTTCAGGTCCAACAAMAGGAACCAT TACAGGGAATACAGTAGTAT TCG S A E D T N V S L I L K F S K E L Q P V S S S G P T K G T I T G N T V V F1711I GATGCTCTACCTAAAT TAGGTTCTAAGGAATCTGTAGAG TTTTCTGTAACATTAAAMGGAATTGCTCCAGGAGATGCTCGAGGAGAGGC TATTCTTTCTTCAGACAC TCTAACGD A L P K L G S K E S V E F S V T L K G I A P G D A R G E A I L S S D T L T182 5 GTACCTGTTGCTGATACAGAAAMCACACATGT TTATTAATCTT TTAAMT TTCTTACTTTGAAMGAT TTGTAMACGAAMAGGCCGTCCTAGAAT TTCC TAGGACGGCTT TT TTGTV P V A D T E N T H V *51939 TTCTCTTTCATCGTCACGATTTTCTGCGGATCCCTCC TAGGAAATGTCACAAAA TTAAATTAAMAAT TTTAAT TAATAGTTGAGTCGGT TAAAC TCT TT TGTCT TAAAGTATT T2053 AGAGAAGGAGTTTTCGTATGTCTGGTGAGAACFigure 1. Nucleotide sequence and deduced amino acid sequence of the 60 kDa Cysteine Rich Outer Membrane Protein of Chlamvdia psittaci. The start and stopcodons are underlined and the putative ribosome binding site is doubly underlined. Potential stem loop structures are shown by facing arrows.,.) 1990 Oxford University Press

The nucleotide sequence of the 60kDa cysteine rich outer membrane protein ofChlamydia psittacistrain EAE/A22/M

https://researchrepository.murdoch.edu.au/id/eprint/55190/1/psittaci.pdf

The nucleotide sequence of the 60kDa cysteine rich outer membrane protein ofChlamydia psittacistrain EAE/A22/M

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