These guides are those used by interviewers who conducted interviews around antibiotic use in humans and animals in Bangladesh. Interviews examined antibiotic use and behaviours contributing to antibiotic resistance from the perspective of household members and healthcare providers in one urban and one rural site in Bangladesh. Household interviews were carried out with the household decision-maker or main care-giver (total 48 interviews). Healthcare provider interviews were carried out with a range of qualified and unqualified providers from human and veterinary medicine (total 46 interviews). The interviews gathered information on where people sought treatment for illnesses due to infection, what determined their choice of healthcare provider; factors contributing to antibiotic use in humans and animals; knowledge and understanding of antibiotic resistance. Similar questions about antibiotic prescribing and selling practices were asked of healthcare providers.The associated transcripts are available upon request to the PI at https://doi.org/10.17028/rd.lboro.8953118.v1Further information on the study as well as details on methods and analysis are available under a CC-BY licence at https://doi.org/10.1136/bmjopen-2018-028215 or https://dspace.lboro.ac.uk/2134/36467</div