Not AvailableThe beet armyworm (Spodoptera exigua) and the
rice water weevil (Lissorhoptrus oryzophilus) are two
important insect pests in rice production. To identify
insect-responsive genes in rice, we performed a deep
transcriptome analysis of Nipponbare rice leaves infested
with both beet armyworm and water weevil using massively
parallel signature sequencing (MPSS). Many antisense,
alternative, and novel transcripts were commonly and
specifically induced and suppressed in the infested tissue.
Key genes involved in the defense metabolic pathways such
as salicylic acid and jasmonic acid biosynthesis pathways
were up-regulated in the infested leaves. To validate theMPSS
results, we analyzed the transcriptome of the rice leaves
infested with water weevils using Solexa’s sequencing-bysynthesis
(SBS) method. The MPSS and SBS data were
highly correlated (Pearson’s correlation coefficient=0.85),
and 83% of genes had similar gene expression in both
libraries. Our comprehensive and in-depth survey of the
insect-infested libraries provides a rich genomic resource for
further analyzing the function of key regulatory genes
involved in insect resistance in rice.Not Availabl
