Paramagnetic relaxation enhancement (PRE) is commonly used to speed up spin lattice relaxation time (T1) for rapid data acquisition in NMR structural studies. Consequently, there is significant interest in novel paramagnetic labels for enhanced NMR studies on biomolecules. Herein, we report the synthesis and characterization of a modified poly(styreneâ coâ maleic acid) polymer which forms nanodiscs while showing the ability to chelate metal ions. Cu2+â chelated nanodiscs are demonstrated to reduce the T1 of protons for both polymer and lipidâ nanodisc components. The chelated nanodiscs also decrease the proton T1 values for a waterâ soluble DNA Gâ quadruplex. These results suggest that polymer nanodiscs functionalized with paramagnetic tags can be used to speedâ up data acquisition from lipid bilayer samples and also to provide structural information from waterâ soluble biomolecules.Speeding up data acquisition: Design of a polymer nanodisc containing a DOTA chelator enables the utilization of the PRE effect in studies using lipid nanodiscs. This new technique can be applied to waterâ soluble biomolecules such as Gâ quadruplexes.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/152508/1/anie201910118.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/152508/2/anie201910118-sup-0001-misc_information.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/152508/3/anie201910118_am.pd
