DEVELOPMENT OF HIGH SPEED RRLC METHOD FOR QUANTITATIVE DETERMINATION OF SOME PESTICIDE RESIDUES IN APPLE JUICE

Abstract

Possibilities of normal-phase (NP) and reversed-phase (RP) liquid chromatography methods for determination of methomyl, methidathion and propiconazole residues in apple juice were studied. The investigations were carried out on various analytical columns using RRLC (Rapid Resolution Liquid Chromatography) system coupled with UV-Vis diode array detector. The best conditions for separation and quantitative determination of tested pesticides were obtained using reversed-phase mode and Purospher® Star RP-18 endcapped (30 mm × 4 mm; 3 μm) column. Fast and simple method for direct determination of methidathion and propiconazole in different apple juice matrix was developed. In accordance to European Commission regulation the tested parameters for method validation (selectivity, linearity, precision, limit of detection, limit of quantification and accuracy) were satisfied