RAZINE VITAMINA D U PACIJENATA S OPSTRUKCIJSKOM APNEJOM TIJEKOM SPAVANJU

Abstract

Objectives: Obstructive sleep apnea is pathological condition characterized by recurrent episodes of upper airway collapse during sleep and is associated with multiple metabolic conditions including metabolic syndrome and osteoporosis. The aim of the presented study was to compare 25-dihydroxy vitamin D concentrations and other laboratory parameters between OSA patients and healthy control group. Patients and methods: The study included 30 male patients that were diagnosed with OSA and a control group with 30 gender and BMI matched healthy volunteers. All study subjects underwent physical examination and anthropometric measuring. In detailed interview, information regarding physical activity was taken. Polysomnographic recording (PSG) was used on all study group participants. Automated Chemiluminescence Sandwich Immunoassay (CLIA) method was used to assess 25-dihydroxy vitamin D (Immunodiagnostic Systems, Frankfurt, Germany) levels. Intact parathyroid hormone (iPTH) was analyzed with the electrochemiluminescence immunoassay (ECLIA) method on Roche Cobas e601 (Roche Diagnostics, Mannheim, Germany), while calcium and phosphorus concentrations were determined using standard laboratory procedures. Results: There was no difference in serum concentrations of 25-dihydroxy vitamin D in OSA patients and healthy controls, however aaverage 25-dihydroxy vitamin D levels were low in both groups (49.3 ± 15.8 nmol/L vs. 48.2 ± 17.6 nmol/L, P=0.744). There was a significant negative correlation between 25-dihydroxy vitamin D concentrations and BMI (r=-0.304, P=0.018) and waist circumference (r=-0.359, P=0.004). The correlation between AHI and 25-dihydroxy vitamin D levels was not observed (r=0.024, P=0.900). There was no difference in iPTH, calcium or phosphorus levels between OSA patients and controls. Conclusion: There was no difference in serum concentrations of 25-dihydroxy vitamin D in OSA patients and healthy controls, however serum concentrations of 25-dihydroxy vitamin D were low in both groups. Further studies with a larger number of OSA patients are needed to investigate the relationship between OSA and 25-dihydroxy vitamin D levels.Cilj: Opstrukcijska apneja tijekom spavanja (OSA) je patološko stanje karakterizirano ponavljajućim epizodama kolapsa gornjih dišnih putova tijekom spavanja i povezano je s više metaboličkih stanja uključujući metabolički sindrom i osteoporozu. Cilj ovog istraživanja bio je usporediti koncentracije 25-dihidroksi vitamina D i druge laboratorijske parametre između OSA bolesnika i zdrave kontrolne skupine. Ispitanici i metode: Istraživanje je uključilo 30 muških bolesnika kojima je dijagnosticirana OSA i 30 zdravih dobrovoljaca uparenih prema spolu i indeksu tjelesne mase (ITM). Svi ispitanici su podvrgnuti fizikalnom pregledu i antropometrijskim mjerenjima. Detaljnim intervjuom su prikupljene informacije o tjelesnoj aktivnosti. Svi ispitanici su podvrgnuti polisomnografskom snimanju (PSG). Kemiluminiscencijska imunokemijska analiza (CLIA) metoda je korištena za procjenu razine 25-dihidroksi vitamina D (Immunodiagnostic Systems, Frankfurt, Njemačka). Intaktni paratiroidni hormon (iPTH) analiziran je metodom elektrokemiluminiscencijskog imunotesta (ECLIA) na Roche Cobas e601 (Roche Diagnostics, Mannheim, Njemačka), dok su koncentracije kalcija i fosfora određene standardnim laboratorijskim postupcima. Rezultati: Nije bilo razlike u serumskim koncentracijama 25-dihidroksi vitamina D u OSA bolesnika i zdravih kontrola, međutim, prosječne vrijednosti 25-dihidroksi vitamina D bile su niske u obje skupine (49,3 ± 15,8 nmol/L naprema 48,2 ± 17,6 nmol/L, P=0,744). Opažena je značajna negativne korelacija između koncentracija 25-dihidroksi vitamina D i indeksa tjelesne mase (r =-0,304, P=0,018) i opsega struka (r=-0,359, P=0,004). Korelacija između AHI i razina 25-dihidroksi vitamina D nije uočena (r=0,024, P=0,900). Nije bilo razlike u razinama iPTH, kalcija ili fosfora između bolesnika s OSA-om i kontrola. Zaključci: Nije bilo razlike u serumskim koncentracijama 25-dihidroksi vitamina D u OSA bolesnika i zdravim kontrolama, međutim serumske koncentracije 25-dihidroksi vitamina D bile su niske u obje skupine. Potrebna su daljnja istraživanja s većim brojem OSA pacijenata kako bi se istražio odnos između OSA-e i razine 25-dihidroksi vitamina D