Genotype response to haploid embryo induction with pollination by irradiated pollens in melon, obtaining of dihaploid lines, determination of haploid and diploid plants by different techniques [Kavunda işinlanmiş polen tozlamalari ile haploid embriyo uyartiminda genotip etkisi, dihaploid hatlarin oluşturulmasi, haploid ve diploid bitkilerin degişik yöntemlerle ayrimi]

Abstract

In this study, 18 melon cultivars which belong to 3 different botanical varieties (var. inodorus, var. reticulatus and var. cantalupensis) of Cucumis melo were tested in order to investigate genotypes effect on hapioid embryos induction by pollination with pollens irradiated with Co60 gamma ray. Embryos were obtained from gamma ray. Embryos were obtained from 14 genotypes belong to 3 different botanical varieties and these embryos were regenerated into plantlets using in vitro embryo rescue method. In order to obtain dihaploid plants, in vitro hapioid plantlets were firstly treated with 0.5 % colchidne solution for two hours and then they were cloned with in vitro micro cutting. These dihaploid plants were acclimatizated in greenhouse conditions. The percentage of acclimatization were ranged between 57-63 % . Seeds were obtained by selfing from all plants grown in greenhouse. Ploidy levels of hapioid and dihaploid plants verified using both chromosome counting (n=12) on root cells and flow cytometry on leaf cells. Also the measurement of stomata dimension and counting of chroloplast in guard cells were performed in hapioid and dihaploid plants. The results showed that stoma diameter and length of dihaploids were higher than haploids at the rate of 31 % and 58 % respectively. The number of chloroplast was ranged between 6-8 in haploids and 10-12 in diploids