PubMedID: 17385339The covalent immobilization of bovine liver catalase (CAT) on to florisil via glutaraldehyde was investigated. Optimum immobilization pH and temperature were determined as pH 6.0, 10°C respectively, while the amount of initial CAT per g of carrier and immobilization time was determined as 5 mg g -1 and 120 min, respectively. The Vmax values for free and immobilized CAT were found to be 1.7 × 105 and 2.0 × 104 µmol H2O2, min-1 mg protein-1, respectively, whereas KM values were 33.3 mM and 1722.0 mM respectively. Operational stability was determined by using a stirred batch-type column reactor. Immobilized CAT retained about 40% of its initial activity after 50 uses. It showed higher storage stability than free CAT at 4°C and 25°C. Its storage stability increased with increasing relative humidity (RH) from 0 to 20% of the medium. The highest storage stability was obtained in 20% RH, however, further increase in RH from 40 to 100% significantly decreased the storage stability
