Mitochondrial DNA variation in honey bee (apis mellifera l.) populations from Turkey

Abstract

We have studied mitochondrial (mt) DNA variation in 334 honey bee colonies from 7 different geographic regions of Turkey. We have evaluated Dra I restriction profiles of the CO-I CO-II intergenic region, Hinf-I and Taq-I restriction profiles of the CO-I gene and EcoR-I restriction profiles of the whole mtDNA. We obtained three different mtDNA patterns by EcoR-I digestion. The pattern typical for A. m. carnica/A. m. ligustica predominated throughout Turkey (327 colonies, 97.9%). We observed the pattern common in African subspecies only in Hatay province (6 colonies, 1.8%) and a previously unreported pattern in one colony from Balıkesir province (0.3%). Dra-I restriction analysis of the CO-I CO-II intergenic region yielded seven haplotypes. Haplotype 1 (TrDra-1) was the most common one found in Turkey, whereas haplotype 2 (TrDra-2) was widely distributed in Eastern Anatolia. Based on mitochondrial ND2 sequences taken from two samples collected in each region, bees from Hatay clustered with A. m. lamarckii and A. m. meda (morphological A and O lineages), while bees from central Anatolia clustered within the C morphological lineage group. © 2006 International Bee Research Association.North Atlantic Treaty Organization: CRG.309726The authors would like to thank to all who assisted to this project in various ways. L. Garnery provided insight and guidance in methodology pertaining to the Dra-I test. This project was partially supported by a grant (VHAG -1077) from TUBITAK to MK, by an Integrated Ph.D. Program project grant from TUBITAK to AK, and by a NATO collaborative linkage grant (CRG.309726) to AK, LG, and WS. We also thank to anonymous reviewers for their comments on an earlier version of the manuscript