Croubels, Siska

Goessens, Tess

Goethals, Peter

Lens, Luc

Martel, An

Pasmans, Frank

Spanoghe, Pieter

Vanhaecke, Lynn

English

Ghent University Academic Bibliography

DYNAMIC SCREENING OF AGRICULTURAL CONTAMINANTS IN FRESHWATER ECOSYSTEMSAS PART OF AMPHIBIAN BIODIVERSITY CONSERVATION 1 DEPARTMENT OF PHARMACOLOGY, TOXICOLOGY AND BIOCHEMISTRY, FACULTY OF VETERINARY MEDICINE, GHENT UNIVERSITY, MERELBEKE, BELGIUM2 DEPARTMENT OF VETERINARY PUBLIC HEALTH AND FOOD SAFETY, FACULTY OF VETERINARY MEDICINE, GHENT UNIVERSITY, MERELBEKE, BELGIUM3 DEPARTMENT OF PLANTS AND CROPS, FACULTY OF BIOSCIENCE ENGINEERING, GHENT UNIVERSITY, GHENT, BELGIUMGoessens Tess1, Vanhaecke Lynn2, Spanoghe Pieter3, Lens Luc4, Goethals Peter5, Martel An6, Pasmans Frank6 and Croubels Siska1Contact:Tess.Goessens@ugent.beSiska.Croubels@ugent.behttps://www.ugent.be/di/ftb/enLaboratory of Pharmacology and Toxicology (GLP compliant)Acknowledgement: this research is supported by the Special Research Fund of Ghent University grant numberBOF16-GOA-024.08General conclusionA variety of multi-residue analytical methods was applied for the simultaneous detection of pesticides (n=93), antimicrobial drug residues (n=46), mycotoxins (n=21),coccidiostats (n=12) heavy metals (n=8) and anthelmintics (n=3) in pond water resulting from 26 amphibian breeding ponds selected across Flanders, Belgium. Mostabundant compounds for each group of contaminants were zinc (Cmax = 333 ng mL-1), terbuthylazine (Cmax = 39 ng mL-1), levamisole (Cmax = 5.7 ng mL-1), 4-epioxytetracycline (Cmax = 0.4 ng mL-1), amprolium (Cmax = 0.03 ng mL-1) and enniatin B (Cmax= 0.01 ng mL-1). Overall, the concentrations of enniatin B and 4-epioxytetracycline were relatively stable during the sampling period. Zinc concentrations were significantly lower in May in comparison with March, April and June: 30versus 62, 66 and 45 ng mL-1, respectively (with a p-value of < 0.05). These findings could be related to a reduced application of pig manure, associated with higher zincconcentrations, on the surrounding agricultural fields in combination with higher precipitation in May.4DEPARTMENT OF BIOLOGY, FACULTY OF SCIENCES, GHENT UNIVERSITY, GHENT, BELGIUMIntroductionObjectiveThe overall objective of this study was to assess the level of contamination in amphibian breeding ponds and the general evolution over time. MethodsIn recent years the presence of agricultural contaminants in the aquatic environment has gained more interest. Research has shown negative effects on the fitness ofaquatic amphibians and zooplankton as part of the aquatic food web. Furthermore, because most amphibians reside in aquatic habitats during breeding season andbecause they have a highly permeable skin, these species tend to be more exposed to environmental toxins than other aquatic and terrestrial vertebrates. Additionally,increasing anthropogenic pressure and agricultural pollution is affecting disease dynamics of chytridiomycosis in amphibians. The latter causing major populationdeclines and even global extinction of species. In this study, a variety of a multi-residue analytical methods was applied for the simultaneous detection of pesticides(n=93), antimicrobial drug residues (n=46), mycotoxins (n=21), coccidiostats (n=12), heavy metals (n=8) and anthelmintics (n=3) in pond water resulting from 26amphibian breeding ponds selected across Flanders, Belgium. Ponds were sampled monthly over a period of 4 months from March until June.Results Field Sampling Class Concentration range (ng mL-1) Most abundant compound Heavy metals 0.206-333 zincAntimicrobial drug residues 0.003-0.422 4-epioxytetracyclinePesticides 0.002-38.7 terbuthylazineMycotoxins 0.001-0.007 enniatin BCoccidiostats 0.005-0.029 amproliumAnthelmintics 0.003-5.700 levamisoleTable 7: Contaminants detected in pond water during the sampling campaign of March untill June 2019.Fig. 1: Sampling locations selected across East Flanders, Belgium including ponds in Zottegem (n=7), Brakel (n=11), Lierde (n=1), Geraardsbergen (n=4) , Maarkedal (n=2) and Zwalm (n=1).  Fig. 3: 4-epioxytetracycline detection in pond BRA12 (Brakel, Belgium). Table 1: Overview of the 93 pesticides included in the screening method. DDD* = dichlorodiphenyldichloroethane DDT* =  dichlorodiphenyltrichloroethane DDE* = dichlorodiphenyldichloroethylene. 5DEPARTMENT OF ANIMAL SCIENCES AND AQUATIC ECOLOGY, FACULTY OF BIOSCIENCE ENGINEERING, GHENT UNIVERSITY, GENT, BELGIUM6DEPARTMENT OF PATHOLOGY, BACTERIOLOGY AND AVIAN DISEASES, FACULTY OF VETERINARY MEDICINE, GHENT UNIVERSITY, MERELBEKE, BELGIUMTable 2: Overview of the 46 antimicrobial drug residues included in the screening method. *diketopiperazine = amoxicillin-diketopiperazine-2’,5’-dioneTable 3: Overview of the 21 mycotoxinsincluded in the screening method. Table 4: Overview of the 12 coccidiostats included in the screening method. Table 6: Overview of the 3 anthelmintics included in thescreening method. acephate chlorpyrifos ethoprophos lindaan pendimethanil tebufenozideacetamiprid chlorthalonil fenamiphos linuron pirimicarb tebuthiuronalachlor cyflufenamid fenbuconazole malathion prochloraz temephosaldrin cymoxanil fenitrothion metalaxyl profenofos terbuthylazineamethryn cypermethrin fenoxycarb methiocarb propanil thiabendazoleazoxystrobine difenconazole fenpropimorf methomyl propiconazole thiaclopridbentazon DDD* fludioxonil methoxychlor propoxur thiametoxambifenthrin DDE* heptachlorobenzeen methsulfuron methyl prosulfocarb thifensulfuronbitertanol diazianon hexachlorobenzeen metribuzin pyraclostrobin thiodicarbboscalid dieldrin hexaconazole monocrotophos pyrazosulfuron ethyl thiofanate-methylbutachlor dimethoate hexythiazox nicosulfuron pyrimethanil triademinolcadusafos dimethomorph imazalil o,p'-DDT* spirodiclofen triazophoscaptan diuron imidacloprid oxamyl spiroxamine trifloxystrobinecarbaryl endosulfan iprodione p,p'-DDT* spinosad Acarbendazim endrin kresoxim methyl parathion spinosad Dcarbofuran epoxiconazole lambda-cyhalothrin penconazole tebuconazole4-epichlortetracycline florphenicol sarafloxacin4-epioxytetracycline flumequine sulfachlorpyridazine4-epitetracycline furaltadon sulfadiazinecefapirin furazolidon sulfadimethoxineceftiofur lincomycin sulfadoxinecefquinome marbofloxacin sulfamethoxazolechloramphenicol nalidixic acid sulfamerazinechlortetracycline nifursol sulfamethazineciprofloxacin nitrofurantoïn sulfathiazolecloxacillin norfloxacin tiamulindanofloxacin ofloxacin tilmicosindifloxacin oxacillin tetracyclinediketopiperazine* oxolinic acid trimethoprimdoxycycline oxytetracycline tylosinenrofloxacin penicillin Gerythromycin A penicillin V15-acetyldeoxynivalenol deoxynivalenol3-acetyldexoxynivalenol enniatin Aaflatoxin B1 enniatin A1aflatoxin M1 enniatin Balternariol methylether enniatin B1α-zearalanol HT2-toxinα-zearalenol ochratoxin Aβ-zearalanol T2-toxinβ-zearalenol zearalanonebeauvericin zearalenonedeepoxy-deoxynivalenol 4,4’-dinitrocarbanilide monensinamprolium narasindiclazuril robenidinhalofuginone salinomycinlasalocid semduramicinmaduramicin toltrazurilflubendazoleivermectinlevamisoleSolid Phase Extraction: Oasis®HLB or Sep-Pak®  UHPLC-XevoTM MS/MS AgilentTM GC-ECD AgilentTM ICP-OESTable 5: Overview of the 8 heavy metals included in the screening method. arsenic leadcadmium mercurychromium nickelcopper zincUHPLC-Q-OrbitrapTM HRMS Filter Glas Microfiber WhatmannTMGrab SamplesFig. 2: Box-plots of the concentration range (ng mL-1) of zinc (a), enniatin B (b) and 4-epixoxytetracycline (c) during the months of March, April, May and June. Within the box plot chart the crosspieces of each box plot represent (from top to the bottom) maximum, upper-quartile, median (black bar), lower-quartile and minimum values.b ca

Dynamic screening of agricultural contaminants in freshwater ecosystems as part of amphibian biodiversity conservation

Houbraken, M.

De Rop, Jasmine

ENVIRONMENTAL SCREENING OF AGRICULTURAL CONTAMINANTS IN FRESH WATER ECOSYSTEMS AS PART OF AMPHIBIAN BIODIVERSITY CONSERVATION   T. GOESSENS*1, M. HOUBRAKEN2, J. DE ROP2, P. GOETHALS3, F. PASMANS4, P. SPANOGHEa2, S. CROUBELS a1 1Ghent University, Department of Pharmacology, Toxicology and Biochemistry, Laboratory of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Merelbeke, Belgium (a shared senior author) 2Ghent University, Department of Plants and Crops, Laboratory of Crop Protection Chemistry, Faculty of Bioscience Engineering, Gent, Belgium 3Ghent University, Department of Applied Ecology and Environmental Biology, Laboratory of Environmental Toxicology and Aquatic Ecology, Faculty of Bioscience Engineering, Gent, Belgium 4Ghent University, Department of Pathology, Bacteriology and Avian Diseases, Laboratory of Veterinary Bacteriology and Mycology, Faculty of Veterinary Medicine, Merelbeke, Belgium   In recent years the presence of agricultural contaminants such as pesticides, mycotoxins and heavy metals in the aquatic environment have gained more interest as research has shown negative effects on fertility and vitality of aquatic amphibians as well as growth impairment of microorganisms that are important for the ecological balance of the food chain. Furthermore, because most amphibians are exposed to aquatic habitats at one point during their life cycle, for example during breeding season, and because they have a highly permeable skin, these species tend to be more sensitive to environmental toxins than other aquatic and terrestrial vertebrates. The overall objective of this project is to assess the contamination of amphibian breeding ponds as part of biodiversity conservation. For this reason, 18 amphibian breeding ponds were selected across East Flanders and sampled at three different locations in the pond for the determination of pesticides, mycotoxins and heavy metals. Pesticides (n=83) and mycotoxins (n=15) were analyzed by a multi-residue method using solid phase extraction (SPE) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Gas chromatography with electron capture detection (GC-ECD) was used to detect the heavily chlorinated or fluorinated pesticides (n=16). Inductively coupled plasma-optical emission spectrometry (ICP-OES) was used for the determination of cadmium, copper, arsenic, chromium, mercury, nickel, lead and zinc. Results of the field sampling will be presented at the conference. This research is supported by the Special Research Fund of Ghent University grant number BOF16-GOA-024.08.  *: corresponding author: tess.goessens@ugent.be1 

Environmental screening of agricultural contaminants in fresh water ecosystems as part of amphibian biodiversity conservation

https://biblio.ugent.be/publication/8641456/file/8641469

Dynamic screening of agricultural contaminants in freshwater ecosystems as part of amphibian biodiversity conservation

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Ghent University Academic Bibliography

Ghent University Academic Bibliography