The paper presents a comparison of birch pollen seasons in 2016 in study sites located in the southern part of Poland: Zielona Gora, Opole, Wroclaw, Sosnowiec, Cracow, Lublin, and Guciow in the Roztocze National Park. The pollen concentrations were measured with the volumetric method using Burkard or Lanzoni pollen samplers. The annual pollen sum was calculated for each measurement site. In 2016, the birch pollen season started at a similar time, i.e. between 4th and 6th April in all the localities. The highest annual sums and maximum pollen concentrations were recorded in Lublin and Guciow. The maximum concentrations of birch pollen were noted from 5th and 15th April, with the highest value in Lublin, i.e. 8573 P/m3 (14.04)