Acha Sagredo, Amelia

Aguirre Urízar, José Manuel

Aguirregaviria, José Ignacio

Ruesga, María T.

A Acha-Sagredo

MT Ruesga

JI Aguirregaviria

JM Aguirre-Urizar

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HPV infection and oral carcinogenesis

Repositori d'Objectes Digitals per a l'Ensenyament la Recerca i la Cultura

e1Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15 (1):e1-2.                                                                                                                                                                           HPV and oral carcinogenesisJournal section: Oral Medicine and Pathology                                                                                                                 doi:10.4317/medoral.15.e1Publication Types: Letter to the EditorHPV infection and oral carcinogenesisAmelia Acha-Sagredo 1, Maria-Teresa Ruesga 1, Jose-Ignacio Aguirregaviria 2, Jose-Manuel Aguirre 11 Oral Medicine and Pathology Unit. Faculty of Medicine and Dentistry. University of the Basque Country EHU. Leioa. Spain2 Department of ENT and Maxillofacial Surgery of San Sebastian Oncological Institute. Guipuzcoa, SpainCorrespondence: Medicina Bucal. Unidad de Patología Oral y Maxilofacial.Facultad de Medicina y Odontología. Universidad del País Vasco EHUBarrio Sarriena s/n Leioa 48940 Vizcaya Spainameliaacha@gmail.com  Received: 02/04/2009Accepted: 20/05/2009Acha-Sagredo A, Ruesga MT, Aguirregaviria JI, Aguirre JM. HPV infec-tion and oral carcinogenesis. Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15 (1):e1-2.      http://www.medicinaoral.com/medoralfree01/v15i1/medoralv15i1p1.pdfArticle Number: 2795            http://www.medicinaoral.com/© Medicina Oral S. L. C.I.F. B 96689336 - pISSN 1698-4447 - eISSN: 1698-6946eMail:  medicina@medicinaoral.com Indexed in: -SCI EXPANDED-JOURNAL CITATION REPORTS-Index Medicus / MEDLINE  /  PubMed                       -EMBASE, Excerpta Medica-SCOPUS-Indice Médico Español                                                        To the Editor,High risk human papilloma viruses (HPV) have been found in head and neck squamous cell carcinomas (HNSCC), particularly in oropharyngeal carcinomas (1). Nevertheless, there are some controversial aspects regarding this issue (2) such as whether the HPV infec-tion is a temporary or a persistent oral infection in these patients.Recently, Chuang et al. (3) have associated the presence of HPV-16 DNA in surveillance salivary rinses with a significant risk for recurrence in HNSCC.We hypothesized that the improved prognosis of many patients with HPV-related oropharyngeal carcinoma is due to the temporary nature of the infection, and there-fore in the absence of lesions no HPV genomic DNA is detected in oral smears.We have analyzed a longitudinal cohort of 47 patients who had been treated for a previous oral squamous cell carcinoma (OSCC) without any precancerous or cancer-ous oral lesion when the oral smear was collected. The group was composed of 29 men and 18 women with a mean age of 62.02 years (45-87 years). Primary OSCC sites were tongue (44.6%), floor of the mouth (31.9%), oropharynx (17%), gingiva (12.7%) and buccal muco-sa (8.5%). Tumours were classified as T1 (53.3%), T2 (37.7%), T3 (2.2%) and T4 (6.6%).A cytological brush (CytobrushTM) was vigorously swabbed over the oral cavity. Cytological samples were centrifuged at 1500 rpm for 15 minutes and washed with phosphate-buffered saline (PBS) to obtain the cellular pellet. DNA was extracted following the clas-sical proteinase-K, phenol/chloroform method and pre-cipitated with ethanol. The detection of HPV genomic DNA was performed using the Papitype kit (Progenie-Molecular S.L, Valencia. Spain). In brief, a polymerase-chain reaction (PCR) targeting the L1 region of the viral genome, harbouring an internal control to avoid false negatives, was carried out. Subsequently, and in HPV+ samples, typing was performed using a restriction frag-ment length polymorphism (RFLP).To our surprise, no HPV genomic DNA was detected in any of the samples. These results agree with other published data (4, 5) which indicate that the HPV infec-tion, when present in oral cancer patients, is temporary and probably related with the presence of a malignant or premalignant lesion.Conscious of the limitations of our study, we think, as other authors (3), that the presence of HPV genomic DNA in oral smears may be used as a biomarker in e2Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15 (1):e1-2.                                                                                                                                                                          HPV and oral carcinogenesisOSCC patients. Additionally, we would like to address that the use of oral smears to detect HPV genomic DNA is an easy and harmless methodology (6, 7).References1. D’Souza G, Kreimer AR, Viscidi R, Pawlita M, Fakhry C, Koch WM, et al. Case-control study of human papillomavirus and oropha-ryngeal cancer. N Engl J Med. 2007;356:1944-56. 2. Pintos J, Black MJ, Sadeghi N, Ghadirian P, Zeitouni AG, Viscidi RP, et al. Human papillomavirus infection and oral cancer: a case-control study in Montreal, Canada. Oral Oncol. 2008;44:242-50. 3. Chuang AY, Chuang TC, Chang S, Zhou S, Begum S, Westra WH, et al. Presence of HPV DNA in convalescent salivary rinses is an ad-verse prognostic marker in head and neck squamous cell carcinoma. Oral Oncol. 2008;44:915-9. 4. Schwartz SM, Daling JR, Doody DR, Wipf GC, Carter JJ, Mad-eleine MM, et al. Oral cancer risk in relation to sexual history and evidence of human papillomavirus infection. J Natl Cancer Inst. 1998;90:1626-36. 5. Herrero R, Castellsagué X, Pawlita M, Lissowska J, Kee F, Balaram P, et al. Human papillomavirus and oral cancer: the Inter-national Agency for Research on Cancer multicenter study. J Natl Cancer Inst. 2003;95:1772-83. 6. Acha A, Ruesga MT, Rodríguez MJ, Martínez de Pancorbo MA, Aguirre JM. Applications of the oral scraped (exfoliative) cytol-ogy in oral cancer and precancer. Med Oral Patol Oral Cir Bucal. 2005;10:95-102. 7. Trullenque-Eriksson A, Muñoz-Corcuera M, Campo-Trapero J, Cano-Sánchez J, Bascones-Martínez A. Analysis of new diagnostic methods in suspicious lesions of the oral mucosa. Med Oral Patol Oral Cir Bucal. 2009;14:E210-6. AcknowledgementsResearch granted by: Carlos III Health Institute – FIS (PI021271) and Department of Education, Universities and Research, Government of the Basque Country (IT-192-07).

HPV infection and oral carcinogenesis

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Repositori d'Objectes Digitals per a l'Ensenyament la Recerca i la Cultura