Chambers HF

Clinical and Laboratory Standards Institute

Duthie ES

Firczuk M

Ingavale SS

Kaneda S

Komatsuzawa H

Novick RP

Odintsov SG

Oshida T

Projan SJ

Sugai M

Yarwood JM

ABSTRACT
          
            Autolysis plays an essential role in bacterial cell division and lysis with β-lactam antibiotics. Accordingly, the expression of autolysins is tightly regulated by several endogenous regulators, including ArlRS, a two component regulatory system that has been shown to negatively regulate autolysis in methicillin-sensitive
            Staphylococcus aureus
            (MSSA) strains. In this study, we found that inactivation of
            arlRS
            does not play a role in autolysis of methicillin-resistant
            S. aureus
            (MRSA) strains, such as community-acquired (CA)-MRSA strains USA300 and MW2 or the hospital-acquired (HA)-MRSA strain COL. This contrasts with MSSA strains, including Newman, SH1000, RN6390, and 8325-4, where autolysis is affected by ArlRS. We further demonstrated that the striking difference in the roles of
            arlRS
            between MSSA and MRSA strains is not due to the methicillin resistance determinant
            mecA
            . Among known autolysins and their regulators, we found that
            arlRS
            represses
            lytN
            , while no effect was seen on
            atl
            ,
            lytM
            , and
            lytH
            expression in both CA- and HA-MRSA strains. Transcriptional-fusion assays showed that the
            agr
            transcripts, RNAII and RNAIII, were significantly more downregulated in the
            arlRS
            mutant of MW2 than the MSSA strain Newman. Importantly, provision of
            agr
            RNAIII in
            trans
            to the MW2
            arlRS
            mutant via a multicopy plasmid induced autolysis in this MRSA strain. Also, the autolytic phenotype in the
            arlRS
            mutant of MSSA strain Newman could be rescued by a mutation in either
            atl
            or
            lytM
            . Together, these data showed that ArlRS impacts autolysis differently in MSSA and MRSA strains.
          </jats:p

Guido Memmi

Dhanalakshmi R. Nair

Ambrose Cheung

Crossref

Journal of Bacteriology

Role of ArlRS in Autolysis in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus Strains

Role of ArlRS in Autolysis in Methicillin-Sensitive and Methicillin-Resistant Staphylococcus aureus Strains

Abstract

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