University of Zagreb. Faculty of Science. Department of Biology.
Abstract
Prometrin spada u skupinu triazinskih herbicida (s-triazina), jednu od najčešće upotrebljavanih skupina herbicida koji su postojani u tlu i vodenom sedimentu. Cilj istraživanja je bio pratiti toksikodinamiku herbicida prometrina u krvi miševa (in vivo) kroz vremenski period od 24h. Miševima je prometrin apliciran oralno, a za utvrđivanje koncentracije prometrina u uzorcima krvi korištena je metoda vezanog sustava plinske kromatografije-spektrometrije masa (GCMS). Prometrin je zabilježen u svim promatranim vremenskim razmacima tijekom 24 sata sa relativno niskom koncentracijom u krvi miševa u odnosu na ukupnu unesenu dozu. Rezultati pokazuju da postoje razlike između mužjaka i ženki u koncentraciji prometrina sve do četvrtog sata nakon oralnog unosa prometrina. Nakon četvrtog sata razlika više nije statistički značajna te je koncentracija prometrina u krvi podjednaka i u muškom i u ženskom organizmu. Iako prometrin nakon 24h ostaje u krvi u niskim koncentracijama, to ukazuje na činjenicu da se u potpunosti ne eliminira i ima potencijal postepenog nakupljanja u organizmu.Prometryne is a member of triazine herbicide group (s-triazines) which make one of the most common herbicide groups and are persistent in soil and water sediment. The aim of this study was to describe toxicodynamics of prometryne in the mice blood (in vivo) during 24 hour time-period. Mice were given a single acute dose per os and the detection of prometryne blood concentration was done with gas chromatography-mass spectrometry (GCMS) method. Prometryne was detected in all analyzed times over 24 hours with relatively low percentage in contrast to the entry dose. Results indicate a significant statistical difference between sexes until the 4th hour from prometryne oral entry. After 4th hour the concentration of prometryne in male and female mice becomes equal and it is no longer statistically significant. Although prometryne is detected with low concentrations after 24 hours, this shows that it is not completely eliminated from the organism and might build up and increase in total blood concentration
