Bester-van der Merwe, A

Dube, B

Dzama, Kennedy

Letuka, M

Maphol, NO

Matika, Oswald

Nesengani, LT

Wragg, David

English

Edinburgh Research Explorer

Gene expression patterns observed from the skin of selected cattle breeds infested with Amblyomma hebraeum ticks

Background: Ticks and tick-borne diseases are a global problem and considered as one of the major challenges to livestock health and performance. Ticks affect 80% of the cattle population across the world with an estimated economic loss of USD $20 – 30 billion per/year worldwide. Numerous tick control strategies have been employed in Southern Africa including the application of acaricides. However, development of resistance to chemicals by ticks limits the effectiveness of this control strategy. Breeding for resistance or tolerance offers a complementary approach that is sustainable in the long term. This study aimed to identify genes that mediate immune responses in tick-tolerant Nguni, tick-susceptible Angus, and F2 Nguni X Angus cattle, infested with Amblyomma hebraeum ticks.Methods: Cattle were phenotyped for resistance to Amblyomma hebraeum ticks as determined by the total number of engorged ticks after a period of 10 days. Skin biopsy samples were collected before infestation, defined as day 1- and 10-days post-infestation. RNA was isolated from the skin biopsies and sequenced to identify differentially expressed genes between these two time points in resistant and susceptible animals.Results: Five genes (PHKG1, PHKA1, CAMK21, NOS2, HRC) were significantly differentially expressed between the susceptible and resistant cattle. Four of these five genes were highly expressed (significant) in the susceptible group before tick infestation. CAMK21 was found to be highly expressed in the resistantt group after tick infestation. HRC was found to be highly (significant) expressed in the susceptible group before tick infestation, but only highly expressed in resistant group after tick infestation.Conclusion: This research reports differential gene expression in the skin following tick bites, thus providing preliminary data concerning the underlying mechanisms associated with the immune response. <br/

     Edinburgh Research Explorer                                      Gene expression patterns observed from the skin of selectedcattle breeds infested with Amblyomma hebraeum ticksCitation for published version:Letuka, M, Wragg, D, Bester-van der Merwe, A, Dzama, K, Nesengani, LT, Matika, O, Dube, B & Maphol,NO 2022, 'Gene expression patterns observed from the skin of selected cattle breeds infested withAmblyomma hebraeum ticks', Plant and Animal Genome XXIX Conference(January 8-12, 2022), UnitedStates, 8/01/22 - 12/01/22.Link:Link to publication record in Edinburgh Research ExplorerDocument Version:Publisher's PDF, also known as Version of recordGeneral rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s)and / or other copyright owners and it is a condition of accessing these publications that users recognise andabide by the legal requirements associated with these rights.Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorercontent complies with UK legislation. If you believe that the public display of this file breaches copyright pleasecontact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately andinvestigate your claim.Download date: 08. Jun. 2022Title: Gene expression patterns observed from the skin of selected cattle breeds infested with Amblyomma hebraeum ticks M Letuka1, D Wragg2,3, A Bester-van der Merwe1, K Dzama1, LT Nesengani4, O Matika2,3, B Dube5 and NO Maphol4 1Department of Animal Sciences, University of Stellenbosch, South Africa 2Centre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Easter Bush Campus, EH25 9RG, UK 3The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush Campus, EH25 9RG, UK 4Department of Agriculture and Animal Health, University of South Africa, South Africa 5Agricultural Research Council, South Africa, Private X2, Irene, 0062  ABSTRACT BODY  Background: Ticks and tick-borne diseases are a global problem and considered as one of the major challenges to livestock health and performance. Ticks affect 80% of the cattle population across the world with an estimated economic loss of USD $20 – 30 billion per/year worldwide. Numerous tick control strategies have been employed in Southern Africa including the application of acaricides. However, development of resistance to chemicals by ticks limits the effectiveness of this control strategy. Breeding for resistance or tolerance offers a complementary approach that is sustainable in the long term. This study aimed to identify genes that mediate immune responses in tick-tolerant Nguni, tick-susceptible Angus, and F2 Nguni X Angus cattle, infested with Amblyomma hebraeum ticks. Methods: Cattle were phenotyped for resistance to Amblyomma hebraeum ticks as determined by the total number of engorged ticks after a period of 10 days. Skin biopsy samples were collected before infestation, defined as day 1- and 10-days post-infestation. RNA was isolated from the skin biopsies and sequenced to identify differentially expressed genes between these two time points in resistant and susceptible animals. Results: Five genes (PHKG1, PHKA1, CAMK21, NOS2, HRC) were significantly differentially expressed between the susceptible and resistant cattle. Four of these five genes were highly expressed (significant) in the susceptible group before tick infestation. CAMK21 was found to be highly expressed in the resistantt group after tick infestation. HRC was found to be highly (significant) expressed in the susceptible group before tick infestation, but only highly expressed in resistant group after tick infestation. Conclusion: This research reports differential gene expression in the skin following tick bites, thus providing preliminary data concerning the underlying mechanisms associated with the immune response.   Key words: Ticks resistant, Gene expression, Angus, Nguni  

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Gene expression patterns observed from the skin of selected cattle breeds infested with Amblyomma hebraeum ticks

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