Fasahath Husain

Hannah M. Wexler

Justin Hsi

Rosemary Meggersee

Valerie Abratt

Yaligara Veeranagouda

ABSTRACT
          
            Two multidrug-resistant
            Bacteroides fragilis
            clinical isolates contain and express a novel
            nim
            gene,
            nimJ
            , that is not recognized by the “universal”
            nim
            primers and can confer increased resistance to metronidazole when introduced into a susceptible strain on a multicopy plasmid. HMW615, an appendiceal isolate, contains at least two copies of
            nimJ
            on its genome, while HMW616, an isolate from a patient with sepsis, contains one genomic copy of
            nimJ. B. fragilis
            NimJ is phylogenetically closer to
            Prevotella baroniae
            NimI and
            Clostridium botulinum
            NimA than to the other known
            Bacteroides
            Nim proteins. The predicted protein structure of NimJ, based on fold recognition analysis, is consistent with the crystal structures derived for known Nim proteins, and specific amino acid residues important for substrate binding in the active site are conserved. This study demonstrates that the “universal”
            nim
            primers will not detect all
            nim
            genes with the ability to confer metronidazole resistance, but
            nimJ
            alone cannot account for the very high metronidazole MICs of these resistant clinical isolates.
          </jats:p

Crossref

Antimicrobial Agents and Chemotherapy

Two Multidrug-Resistant Clinical Isolates of Bacteroides fragilis Carry a Novel Metronidazole Resistance
            <i>nim</i>
            Gene (
            <i>nimJ</i>
            )

Two Multidrug-Resistant Clinical Isolates of Bacteroides fragilis Carry a Novel Metronidazole Resistance
                    nim

Two Multidrug-Resistant Clinical Isolates of Bacteroides fragilis Carry a Novel Metronidazole Resistance nim

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