AL García-Basteiro

CF Hanrahan

CM Denkinger

DJ Dhasmana

E López-Varela

G Yağmur

H Getahun

KR Steingart

L Maynard-Smith

LE Scott

M Bates

M Espasa

M Uplekar

MP Nicol

P Castillo

Padmini Salgame

PP Banada

Q Bassat

R Hernández-Pando

SD Lawn

T Iwamoto

VB Patel

Y Gu

Scientific Reports

AbstractThe extent to which the Xpert MTB/RIF (Gene Xpert) contributes to tuberculosis (TB) diagnosis in samples other than sputum and cerebrospinal fluid remains uncertain. We aimed to assess the role of Xpert MTB/RIF for detecting M. tuberculosis in post-mortem tissues. We conducted a study among 30 complete diagnostic autopsies (CDA) performed at the Maputo Central Hospital (Mozambique). Lung tissues were screened for TB in all cases. In addition other tissues were tested when compatible lesions were identified in the histological exam. We used in-house real time PCR and LAMP assays to confirm the presence of M. tuberculosis DNA. The diagnosis of tuberculosis at death was established based on microbiological and histopathological results. Eight out of 30 cases (26.7%) were diagnosed of tuberculosis. Xpert had a sensitivity to detect TB in lung tissue of 87.5% (95% CI 47.3–99.7) and a specificity of 95.7% (95% CI: 78.1–99.9). In-house DNA amplification methods and Xpert showed 93.6% concordance for lung tissue and 100% concordance for brain and liver tissues. The final cause of death was attributable to tuberculosis in four cases. Xpert MTB/RIF may represent a valuable, easy-to perform technique for post-mortem TB diagnosis.</jats:p

Alberto L. García-Basteiro

Mamudo R. Ismail

Carla Carrilho

Esperança Ussene

Paola Castillo

Dércio Chitsungo

Cristina Rodríguez

Lucília Lovane

Andrea Vergara

Elisa López-Varela

Inacio Mandomando

Cesaltina Lorenzoni

Jaume Ordi

Clara Menéndez

Quique Bassat

Miguel J. Martínez

Crossref

The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues

The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues

Abstract

Similar works

Full text

Available Versions

Crossref