Ahn K. S.

Coskun-Ari F. F.

Duggin I. G.

Franks A. H.

Kuempel P. L.

Schneider A.-M.

Smith M. T.

Weiss A. S.

ABSTRACT
          
            The
            Bacillus subtilis
            merodiploid strain GSY1127 contains a large nontandem duplication of a portion of its chromosome within its left (anticlockwise) replication segment. This causes displacement of the replication terminus region to a noticeably asymmetric location relative to
            oriC
            . The utilization of the subsidiary replication terminators,
            TerIII
            and
            TerV
            , in the merodiploid strain has been compared with that in
            B. subtilis
            168. It is shown that
            TerIII
            is utilized to a significant extent in GSY1127 and that
            TerV
            is used only marginally at the most. Neither of these terminators is used to a measurable extent in the 168 strain. It is concluded that
            TerIII
            and
            TerV
            do indeed function as backups to the major terminator
            TerI
            , as has been generally thought. It is further concluded that, in the 168 strain, the vast majority of clockwise forks are arrested at the highly efficient
            TerI
            terminator, with fork fusion between the approaching forks occurring frequently while the clockwise fork is stationary at
            TerI
            .
          </jats:p

A. A. Griffiths

R. G. Wake

Crossref

Journal of Bacteriology

Utilization of Subsidiary Chromosomal Replication Terminators in
            <i>Bacillus subtilis</i>

Utilization of Subsidiary Chromosomal Replication Terminators in Bacillus subtilis

Utilization of Subsidiary Chromosomal Replication Terminators in Bacillus subtilis

Abstract

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