Alexandra Malachova

Cindy Lorenz

D. Cullen

Dietmar Haltrich

Elisabeth Varga

European Commission

Franz Berthiller

Gerhard Adam

Herbert Michlmayr

Nhung Thi Nguyen

ABSTRACT
          
            Glycosylation plays a central role in plant defense against xenobiotics, including mycotoxins. Glucoconjugates of
            Fusarium
            toxins, such as deoxynivalenol-3-
            O
            -β-
            d
            -glucoside (DON-3G), often cooccur with their parental toxins in cereal-based food and feed. To date, only limited information exists on the occurrence of glucosylated mycotoxins and their toxicological relevance. Due to a lack of analytical standards and the requirement of high-end analytical instrumentation for their direct determination, hydrolytic cleavage of β-glucosides followed by analysis of the released parental toxins has been proposed as an indirect determination approach. This study compares the abilities of several fungal and recombinant bacterial β-glucosidases to hydrolyze the model analyte DON-3G. Furthermore, substrate specificities of two fungal and two bacterial (
            Lactobacillus brevis
            and
            Bifidobacterium adolescentis
            ) glycoside hydrolase family 3 β-glucosidases were evaluated on a broader range of substrates. The purified recombinant enzyme from
            B. adolescentis
            (
            Ba
            Bgl) displayed high flexibility in substrate specificity and exerted the highest hydrolytic activity toward 3-
            O
            -β-
            d
            -glucosides of the trichothecenes deoxynivalenol (DON), nivalenol, and HT-2 toxin. A
            
              K
              m
            
            of 5.4 mM and a
            V
            max
            of 16 μmol min
            −1
            mg
            −1
            were determined with DON-3G. Due to low product inhibition (DON and glucose) and sufficient activity in several extracts of cereal matrices, this enzyme has the potential to be used for indirect analyses of trichothecene-β-glucosides in cereal samples.
          </jats:p

Crossref

Applied and Environmental Microbiology

A Versatile Family 3 Glycoside Hydrolase from Bifidobacterium adolescentis Hydrolyzes β-Glucosides of the Fusarium Mycotoxins Deoxynivalenol, Nivalenol, and HT-2 Toxin in Cereal Matrices

A Versatile Family 3 Glycoside Hydrolase from Bifidobacterium adolescentis Hydrolyzes β-Glucosides of the Fusarium Mycotoxins Deoxynivalenol, Nivalenol, and HT-2 Toxin in Cereal Matrices

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