Artsimovitch I

Bailey MJ

Cheville NF

Gao S

Grebe TW

Harel J

Harry EG

La Ragione RM

Landraud L

Lindberg AA

Mokady D

Nagy G

Nemeth J

Nolan LK

Pourbakhsh SA

Stevens MP

Toth TE

ABSTRACT
          
            Avian pathogenic
            Escherichia coli
            (APEC) infection causes avian colibacillosis, which refers to any localized or systemic infection, such as acute fatal septicemia or subacute pericarditis and airsacculitis. The RfaH transcriptional regulator in
            E. coli
            is known to regulate a number of phenotypic traits. The direct effect of RfaH on the virulence of APEC has not been investigated yet. Our results showed that the inactivation of
            rfaH
            significantly decreased the virulence of APEC E058. The attenuation was assessed by
            in vivo
            and
            in vitro
            assays, including chicken infection assays, an ingestion and intracellular survival assay, and a bactericidal assay with serum complement. The virulence phenotype was restored to resemble that of the wild type by complementation of the
            rfaH
            gene in
            trans
            . The results of the quantitative real-time reverse transcription-PCR (qRT-PCR) analysis and animal system infection experiments indicated that the deletion of
            rfaH
            correlated with decreased virulence of the APEC E058 strain.
          </jats:p

Qingqing Gao

Huiqing Xu

Xiaobo Wang

Debao Zhang

Zhengqin Ye

Song Gao

Xiufan Liu

Crossref

Journal of Bacteriology

RfaH Promotes the Ability of the Avian Pathogenic Escherichia coli O2 Strain E058 To Cause Avian Colibacillosis

RfaH Promotes the Ability of the Avian Pathogenic Escherichia coli O2 Strain E058 To Cause Avian Colibacillosis

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