A Dai

A Dewan

B Yao

C Holtze

E Brouzes

FG Acién

GM Whitesides

HS Kim

J Lee

J Nam

J Pan

JFB Mitchell

K Ullah

K Zhang

L Rodolfi

M Hannon

M Hosokawa

M Yu

MA Chia

ME Hong

MH Langholtz

MK Aalst van

P Parupudi

R Sayre

RA Vijayendran

RJ Best

SA Scott

W Klinthong

X Deng

Y Chisti

Y Jo

Y Malhi

YJ Sung

Z Guan

ÉC Francisco

Scientific Reports

AbstractMicroalgae – unicellular photosynthetic organisms – have received increasing attention for their ability to biologically convert CO2 into valuable products. The commercial use of microalgae requires screening strains to improve the biomass productivity to achieve a high-throughput. Here, we developed a microfluidic method that uses a magnetic field to separate the microdroplets containing different concentrations of microalgal cells. The separation efficiency is maximized using the following parameters that influence the amount of lateral displacement of the microdroplets: magnetic nanoparticle concentration, flow rate of droplets, x- and y-axis location of the magnet, and diameter of the droplets. Consequently, 91.90% of empty, 87.12% of low-, and 90.66% of high-density droplets could be separated into different outlets through simple manipulation of the magnetic field in the microfluidic device. These results indicate that cell density-based separation of microdroplets using a magnetic force can provide a promising platform to isolate microalgal species with a high growth performance.</jats:p

Young Joon Sung

Jaoon Young Hwan Kim

Hong Il Choi

Ho Seok Kwak

Sang Jun Sim

Crossref

Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains

Magnetophoretic sorting of microdroplets with different microalgal cell densities for rapid isolation of fast growing strains

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