Garlet GP

Gaspirc B

Oz HS

R Development Core Team

Skyscan BV

StataCorp

ABSTRACT
          
            Aggregatibacter actinomycetemcomitans
            is a Gram-negative commensal bacterium of the oral cavity which has been associated with the pathogenesis of periodontitis with severe alveolar bone destruction. The role of host factors such as reactive oxygen and nitrogen intermediates in periodontal
            A. actinomycetemcomitans
            infection and progression to periodontitis is still ill-defined. Therefore, this study aimed to analyze the role of NADPH oxidase and inducible nitric oxide synthase (iNOS) in a murine model of
            A. actinomycetemcomitans
            -induced periodontitis. NADPH oxidase-deficient (gp91
            phox
            knockout [KO]), iNOS-deficient (iNOS KO), and C57BL/6 wild-type mice were orally infected with
            A. actinomycetemcomitans
            and analyzed for bacterial colonization at various time points. Alveolar bone mineral density and alveolar bone volume were quantified by three-dimensional micro-computed tomography, and the degree of tissue inflammation was calculated by histological analyses. At 5 weeks after infection,
            A. actinomycetemcomitans
            persisted at significantly higher levels in the murine oral cavities of infected gp91
            phox
            KO mice than in those of iNOS KO and C57BL/6 mice. Concomitantly, alveolar bone mineral density was significantly lower in all three infected groups than in uninfected controls, but with the highest loss of bone density in infected gp91
            phox
            KO mice. Only infected gp91
            phox
            KO mice revealed significant loss of alveolar bone volume and enhanced inflammatory cell infiltration, as well as an increased number of osteoclasts. Our results indicate that NADPH oxidase is important to control
            A. actinomycetemcomitans
            infection in the murine oral cavity and to prevent subsequent alveolar bone destruction and osteoclastogenesis.
          </jats:p

Antje Bast

Helen Kubis

Birte Holtfreter

Silvia Ribback

Heiner Martin

Helen C. Schreiner

Malte J. Dominik

Katrin Breitbach

Frank Dombrowski

Thomas Kocher

Ivo Steinmetz

Crossref

Infection and Immunity

NADPH Oxidase Contributes to Resistance against Aggregatibacter actinomycetemcomitans-Induced Periodontitis in Mice

NADPH Oxidase Contributes to Resistance against Aggregatibacter actinomycetemcomitans-Induced Periodontitis in Mice

Abstract

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