MPN-RealTime PCR による市販鶏肉中のCampylobacter jejuniの定量と分布

Abstract

Quantitative analysis of Campylobacter jejuni by MPN-RealTime PCR was performed in retail chicken meats. At the same time, the important microbiological viable counts (such as, total viable cell, coliform, anaerobic cell counts, etc.) were estimated for searching surrogate indicators of C. jejuni counts. The quantitative range of MPN- RealTime PCR was estimated 0.04~31000 CFU/g, 32/52 of samples (62%) were positive for C. jejuni contamination. However, clear correlation was not observed between the level of contamination of C. jejuni and the viable counts of sanitation indicator bacteria tested by multiple regression analysis.RealTime PCRとMPN法を組み合わせた方法により, 市販鶏肉中のCampylobacter jejuniの定量を試みた. また同時に一般生菌数・大腸菌群数・嫌気性菌数などの汚染指標菌数も同時に測定し, これらの結果とC.jejuniの汚染度との関連性を求めた. C. jejuniの定量範囲は0.04~31000 CFU/g であり, 52検体中32検体 (およそ62% )がC. jejuni 陽性と得られた. この定量結果と汚染指標菌数や様々な平板で出現した集落数などとの関連性の有無を重回帰分析により求めたが, 関連性の認められる因子を見出だすことはできなかった

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