Ashrafzadeh, Anis

Simorgh Research Repository

      داﻧﺸﮕﺎه ﻋﻠﻮم ﭘﺰﺷﻜﻲ و ﺧﺪﻣﺎت ﺑﻬﺪاﺷﺘﻲ درﻣﺎﻧﻲ ﻛﺮﻣﺎن  ﻳﻲدارو ﻋﻠﻮم و يداروﺳﺎز داﻧﺸﻜﺪه    ﻧﺎﻣﻪ دﻛﺘﺮاي ﻋﻤﻮﻣﻲ داروﺳﺎزي ﭘﺎﻳﺎن      ﻋﻨﻮان: ﻫﺎي اﺗﺎﻧﻮﻟﻲ ﻣﺎزو       ﻫﺎي ﺣﺎوي ﻋﺼﺎرهﻓﺮﻣﻮﻻﺳﻴﻮن ﻧﻴﻮزوم   )reivilo .G airotcefni sucreuQ( و ﺑﻮﻣﺎدران   )hcoK .C iismlehliw aellihcA(          وﺑﺮرﺳﻲ ﺧﺼﻮﺻﻴﺎت ﻓﻴﺰﻳﻜﻮﺷﻴﻤﻴﺎﻳﻲ آن ﺑﺎ اﺛﺮات ﺑﺎﻟﻘﻮه ﺿﺪ ﻟﻚ   ﺗﻮﺳﻂ:  اﻧﻴﺲ اﺷﺮف زاده    اﺳﺎﺗﻴﺪ راﻫﻨﻤﺎ:  دﻛﺘﺮ ﭘﻴﺎم ﺧﺰاﺋﻠﻲ  دﻛﺘﺮ ﻓﺮﻳﺒﺎ ﺷﺮﻳﻔﻲ ﻓﺮ         5311ﺷﻤﺎره ﭘﺎﻳﺎن ﻧﺎﻣﻪ:                                                                                 8931ﭘﺎﻳﻴﺰ         Kerman University of Medical Sciences Faculty of Pharmacy  Pharm.D Thesis     Title: Niosome formulation and physicochemical characterization of ethanolic extract of Mazo ( Quercus  infectoria G.Olivier) and Yarrow (Achillea wilhelmsii C. Koch)  as potentially depigmenting agent   By: Anis Ashrafzadeh  Supervisors: Dr. Payam Khazaeli Dr. Fariba Sharififar    Thesis No.: 1135                                                                                             Autumn 2019     ﻓﺎرﺳﻲﺧﻼﺻﻪ  ﻳﻲﻫﺎ ﻮلﻜﻳﻫﺎ وزﻮزومﻴﻧ آﻧﺰﻳﻢ ﺗﻴﺮوزﻳﻨﺎز ﻧﻘﺶ ﻛﻠﻴﺪي در ﺑﻴﻮﺳﻨﺘﺰ ﻣﻼﻧﻴﻦ، رﻧﮕﺪاﻧﻪ ﻣﻬﻢ ﭘﻮﺳﺘﻲ، دارد. :ﻣﻘﺪﻣﻪ ﻦﻳاز ﺑﻬﺘﺮ ﻲﻜﻳ ﻦﻴو ارزان ﻫﺴﺘﻨﺪ. ﻫﻤﭽﻨ ﺪارﻳﭘﺎ ،ﻲﺳﻤﺮﻴﻫﺴﺘﻨﺪ ﻛﻪ ﻧﺴﺒﺘﺎ ﻏ ﻲﻮﻧﻳﺮﻴﻏ يﻫﺎﻛﺘﺎﻧﺖﻓﺎﻣﺘﺸﻜﻞ از ﺳﻮر. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ اﻗﺒﺎل ﻋﻤﻮﻣﻲ در ﺧﺼﻮص ﻨﺪﻳآﻲﻫﺎ ﺑﻪ ﺷﻤﺎر ﻣﭙﻮزومﻴﻟ يﺑﺮا ﻲﻨﻳﮕﺰﻳﺑﻮده و ﺟﺎ ﻳﻲدارو يﻫﺎﺣﺎﻣﻞ ﻛﻨﻨﺪه رﻧﮓ ﭘﻮﺳﺖ، ﻫﺪف اﻳﻦ ﺗﺤﻘﻴﻖ،  ﻓﺮﻣﻮﻻﺳﻴﻮن و ﺑﺮرﺳﻲ ﻓﻴﺰﻳﻜﻮﺷﻴﻤﻴﺎﻳﻲ ﻧﻴﻮزوم ﺣﺎويﻫﺎي روﺷﻦﻓﺮآورده aellihcAﮔﻴﺎه ﺑﻮﻣﺎدران ﺑﺎ ﻧﺎم ﻋﻠﻤﻲ و reivilo.G airotcefni sucreuQﻣﺎزو ﺑﺎ ﻧﺎم ﻋﻠﻤﻲ ﻋﺼﺎره اﺗﺎﻧﻮﻟﻲ   ﻧﺪ. اﻛﻪ درﻣﻄﺎﻟﻌﺎت ﻗﺒﻠﻲ اﺛﺮ ﻣﻬﺎرﻛﻨﻨﺪﮔﻲ ﻗﺎﺑﻞ ﻗﺒﻮﻟﻲ ﺑﺮ آﻧﺰﻳﻢ ﺗﻴﺮوزﻳﻨﺎز ﻧﺸﺎن دادهﺑﺎﺷﺪ ﻣﻲ hcoK.C iismlehliwﺳﺎﻋﺖ ﺻﻮرت  27درﺟﻪ ﺑﻪ ﻣﺪت  08اﺗﺎﻧﻮل ﻣﺎﺳﺮاﺳﻴﻮن ﮔﺮم و ﺑﺎ اﺳﺘﻔﺎده از  ﻲ ﺑﺎ روشﻫﮔﻴﺎ رهﻋﺼﺎﺗﻬﻴﻪ : روشﻣﻘﺪار ﺗﺮﻛﻴﺒﺎت ﻓﻨﻠﻴﻚ ﺗﺎم ﮔﻴﺎه ﺑﺎ اﺳﺘﻔﺎده از ﻣﻨﺤﻨﻲ ﻫﺎ ﺑﺎ اﺳﺘﻔﺎده از ﺗﻌﻴﻴﻦ ﺳﺎزي ﻋﺼﺎرهﮔﺮﻓﺖ. اﺳﺘﺎﻧﺪاردﺎي در دﻣ ﻠﻢﻴﻓﻳﻮﻧﻲ ﺑﺎ روش ﻫﻴﺪراﺗﺎﺳﻴﻮن ﻫﺎي ﻏﻴﺮﻫﺎي ﺳﻮرﻓﺎﻛﺘﺎﻧﺖوزﻳﻜﻮل اﺳﻴﺪ اﻧﺠﺎم ﺷﺪ.ﻴﻚﻛﺎﻟﻴﺒﺮاﺳﻴﻮن ﮔﺎﻟﻫﺎي اﺳﺘﺮ و (08،06،04،02 napSﻫﺎي ﺳﻮرﺑﻴﺘﺎن )ﺑﺎ اﺳﺘﻔﺎده از ﺗﺮﻛﻴﺐ ﻟﻴﭙﻴﺪ ﺣﺎوي اﺳﺘﺮﮔﺮاد درﺟﻪ ﺳﺎﻧﺘﻲ 06ﻫﺎ ﺑﺎ ﻣﻴﻜﺮوﺳﻜﻮپ ﮔﻴﺮي ﻧﻴﻮزوم( آﻣﺎده ﺷﺪﻧﺪ. ﺗﻮاﻧﺎﻳﻲ ﺷﻜﻞ08،06،04،02 neewTاﺗﻴﻠﻦ ﺳﻮرﺑﻴﺘﺎن )اﻛﺴﻲﭘﻠﻲﮔﺮدﻳﺪ.  مﻧﺠﺎا رﻟﻴﺰ يﭘﺮﺗﻮ اشﭘﺮروش  ﺑﺎ ﻫﺎلﻳﻜﻮوزاي ذره ﻧﺎﻟﻴﺰآﻫﺎ  ﺑﺮرﺳﻲ ﺷﺪ و ﻲ آنﻜﻳﺰﻴﻓ يﺪارﻳﭘﺎ ﻧﻮري وﺳﺎزي ﻋﺼﺎره ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺖ. و آزادﺳﺎزي ﻋﺼﺎره ﺑﺎ اﺳﺘﻔﺎده از ﻣﻘﺪار ﻣﺤﺒﻮسﺑﺮﺗﺮ  ﻮنﻴﻓﺮﻣﻮﻻﺳدر   ﺳﻞ اﻧﺘﺸﺎر ﻓﺮاﻧﺰ ارزﻳﺎﺑﻲ ﺷﺪ. ﺳﻴﺪ اﺗﻌﻴﻴﻦ ﮔﺮدﻳﺪ. ﻣﻘﺪار ﺗﺮﻛﻴﺒﺎت ﻓﻨﻠﻴﻚ ﺗﺎم ﻣﻌﺎدل ﮔﺎﻟﻴﻚ % 2و ﺑﻮﻣﺎدران % 3ﮔﻴﺮي ﻣﺎزودرﺻﺪ ﻋﺼﺎرهﻧﺘﺎﻳﺞ: ، 04، 02اﺳﭙﻦ ) يﻫﺎﺳﻮرﻓﺎﻛﺘﺎﻧﺖ ﻫﻤﻪ .ﻣﺤﺎﺳﺒﻪ ﮔﺮدﻳﺪ ، 8/09 و9/47 ()%W/Wﺑﻮﻣﺎدران ﺑﻪ ﺗﺮﺗﻴﺐﻣﺎزو و    %2ﻋﺼﺎره  يﺣﺎو يﻫﺎ ﻮزومﻴﻓﺎﻗﺪ ﻋﺼﺎره و ﻧ يﻫﺎﻮزومﻴﻧ ﻞﻴﺗﺸﻜ ﻳﻲ( ﺗﻮاﻧﺎ08، 06، 04، 02)ﻦﻴ( و ﺗﻮﺋ06،08،   يﺎﻫﻜﻮلﻳوز ،ﻲﻜﺮوﺳﻜﻮﭘﻴﻣﺸﺎﻫﺪه ﻣﺪ. ﻧﺸﺪﻧ ﻞﻴﻋﺼﺎره ﻣﺎزو ﺗﺸﻜ يﺣﺎو يﻫﺎﻮزومﻴﻧ ﻲﺑﻮﻣﺎدران را داﺷﺘﻨﺪ. وﻟﻣﺘﺸﻜﻞ  ﺳﻮﺳﭙﺎﻧﺴﻴﻮن ﻧﻴﻮزوﻣﻲ. ﺷﺪه ﺑﻮدﻧﺪ را ﻧﺸﺎن داد ﻞﻴﺗﺸﻜ ﻓﻴﻠﻢ، ﻮنﻴﺪراﺗﺎﺳﻴﺑﺰرگ و ﻣﺪور را ﻛﻪ ﺑﺎ روش ﻫ. اﻳﻦ ﺷﺪاﻧﺘﺨﺎب ﺑﺮﺗﺮ  ﻮنﻴﺑﻪ ﻋﻨﻮان ﻓﺮﻣﻮﻻﺳ 05/52/52 ﻲ/ﻛﻠﺴﺘﺮول ﺑﺎ ﻧﺴﺒﺖ ﻣﻮﻟ06ﻦﻴ/ﺗﻮﺋ06اﺳﭙﻦاز ﺳﺎزي ﻗﺒﻮﻟﻲ از ﻣﺤﺒﻮس ﻓﺮﻣﻮﻻﺳﻴﻮن ﻧﻴﻮزوﻣﻲ ﺑﺮﺗﺮ ﺗﻬﻴﻪ ﺷﺪه داراي ﭘﺎﻳﺪاري ﻓﻴﺰﻳﻜﻲ ﺑﺎﻻ و درﺻﺪ ﻗﺎﺑﻞ( ﺑﻮد. ﺑﺮرﺳﻲ ﻣﻴﺰان ﻋﺒﻮر ﻋﺼﺎره ﺑﻮﻣﺎدران ﻣﺤﺒﻮس ﻧﻴﻮزوﻣﻲ از ﻏﺸﺎي ﺳﻠﻮﻓﺎن ﻧﺸﺎن داد ﻛﻪ ﭘﺲ %55/9دارو) ﺷﻮد.ﺳﺎزي اﻧﺠﺎم ﻣﻲدرﺻﺪ آزاد 03ﺳﺎﻋﺖ،  4از  ﻦﻳﻨﺪ. اﻫﺴﺘ ﺪارﻳﭘﺎ ﺎرﻴﺑﺴ ،يﺳﺎزو ﻣﺤﺒﻮس ﻮنﻴﻓﺮﻣﻮﻻﺳ ﻨﺪﻳﻓﺮآ ﻲدر ﻃ، ي ﺗﻬﻴﻪ ﺷﺪهﻫﺎﻮزومﻴﻧ :ﮔﻴﺮيﺘﻴﺠﻪﻧو  ﻲﻤﻨﻳﺪ اﺑﺘﻮاﻧﻨ يﭼﻨﺎﻧﭽﻪ ﻣﻄﺎﻟﻌﺎت ﺑﻌﺪ. ﺷﻮﻧﺪﻲﻣ ﺪﻴﻛﻢ ﺗﻮﻟ ﻨﻪﻳو ﺑﺎ ﻫﺰ ﻲﺑﻪ آﺳﺎﻧ ﺪﻳﺟﺪ ﻲرﺳﺎﻧدارو يﻫﺎﺴﺘﻢﻴﺳ  د.ﻓﺮآورده ﻋﺮﺿﻪ ﻧﻤﻮ ﻦﻳﻟﻚ از اﺿﺪ ﻲﻣﻮﺿﻌ يﻫﺎﻮنﻴﻓﺮﻣﻮﻻﺳ ﺘﻮانﻴﻛﻨﻨﺪ، ﻣ ﺪﻳﻴﻓﺮآورده را ﺗﺎ ﻲاﺛﺮﺑﺨﺸ ﺰﻴﻧ  ﻫﺎي ﭘﻮﺳﺘﻲﻟﻚ ﻧﻴﻮزوم، ﺑﻮﻣﺎدران، ﻣﺎزو،اژه ﻫﺎي ﻛﻠﻴﺪي: و               Abstract IIntroduction: Tyrosinase enzyme plays a key role in the biosynthesis of melanin. Niosomes are vesicles composed of non-ionic surfactants that are relatively nontoxic, stable and inexpensive. It is also one of the best drug carriers and is a replacement for liposomes. With regard to the public interest the skin products as whitening agent the aim of this study is the formulation and physicochemical evaluation of niosomes containing ethanolic extract of Mazo (scientific name: Quercus infectoria G. olivier) and Yarrow (scientific name: Achillea wilhelmsii C. Koch), which have shown acceptable inhibitory effects on tyrosinase in previous studies.  Methods: The extract of the plants was prepared by maceration in ethanol 80% for 72 hours. Extracts were standardized by determination of total phenolic compounds of the plant using gallic acid calibration curve. Non-ionic surfactant vesicles by film hydration at 60°C using a lipid compound containing sorbitan esters (Span 20،40،60،80) and polyoxyethylene sorbitan esters (Tween 20،40،60،80 ) were prepared. Vesicles were morphologically studied by optical microscope and their physical stability were evaluated. size analysis of vesicle was carried out by laser light scattering method. In the best formulation, the amount of encapsulation efficiency of the extract was studied. The release of the extract was evaluated by using Franz diffusion cell.  Results: Percentage of Mazo extract was 3% and yarrow 2%. The total phenolic compounds equivalent to gallic acid mazo and yarrow were calculated 9,74 and 8,90) W/ W  )% , respectively. All surfactants of Span (20, 40, 80, 60) and Tween (20, 40, 60, 80) were capable of forming non-extracted niosomes and niosomes containing 2% of yarrow extract. But the niosomes containing the Mazo extract did not form. Microscopical observation showed round and large vesicles which are formed in film hydration method.  The niosome suspension consisting of Span 60 / Tween 60 / cholesterol with a molar ratio of 25/25/50 was selected as   the best formulation. This best niosomic formulation had high physical stability and acceptable percentage of entrapment of extract (55,9%). In the extract release test, 30% of the extract passed through the cellophane membrane after 240 minutes. Conclusion: Prepared niosomes are highly stable during the formulation and encapsulation. These new drug delivery systems are easily and cheaply manufactured. If further studies can confirm the safety and efficacy of the product, topical anti hyperpigmentation formulations of this product may be offered. Key words: Noisome, Mazo, Yarrow, Hyperpigmentation.                   

فرمولاسیون نیوزوم های حاوی عصاره های اتانولی مازو (Achillea wilhelmsii C. Koch) و بومادران (Quercus infectoria G. olivier) وبررسی خصوصیات فیزیکوشیمیایی آن با اثرات بالقوه ضد لک

http://eprints.kmu.ac.ir/32170/1/7548.pdf

فرمولاسیون نیوزوم های حاوی عصاره های اتانولی مازو (Achillea wilhelmsii C. Koch) و بومادران (Quercus infectoria G. olivier) وبررسی خصوصیات فیزیکوشیمیایی آن با اثرات بالقوه ضد لک

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