Not AvailableIn the present study, an experiment was carried out to delineate the lethal concentration of (LC50) zinc nanoparticles
(Zn-NPs) alone and with concurrent to high temperature (34 °C) in Pangasianodon hypophthalmus. The
lethal concentration of Zn-NPs alone and with high temperature was estimated as 21.89 and 19.74 mg/L respectivey
in P. hypophthalmus. The lethal concentration was decided with the help of definite concentration via
16, 18, 20, 22, 24, 26, 28 and 30 mg/L. The Zn-NPs were significantly alter the biochemical and histopathology
of different fish tissues. The stress biomarkers such as oxidative stress (catalase superoxide dismutase and glutathione-
s-transferase, lipid peroxidation) was studied in the liver, gill and kidney tissue, which was noticeable
(p < 0.01) enhanced with higher concentration in both condition (Zn-NPs alone and Zn-NPs-T) in dose dependent
manners. The carbohydrate (lactate dehydrogenase and malate dehydrogenase) and protein metabolic
enzymes (alanine amino transferase and aspartate amino transferase) were also remarkable enhanced
(p < 0.01) with higher concentration of Zn-NPs and Zn-NPs-T. The neurotransmitter (acetylcholine esterase)
activities were significant inhibited (p < 0.01) with exposure to Zn-NPs and Zn-NPs-T and digestive enzymes
such as protease and amylase were non-significant (p > 0.01) with the exposure of Zn-NPs and Zn-NPs-T,
further, lipase were significantly reduced (p < 0.01) with exposure to Zn-NPs and temperature exposure group.
The histopathological alteration were also observed in the liver and gill tissue. The present investigation suggested
that, essential trace elements at higher concentration in acute exposure led to pronounced deleterious
alteration on histopathology and cellular and metabolic activities in fish.Not Availabl
