Not AvailableThe aim of present study was to assess the osmotic fragility and oxidative stress in horse
erythrocytes infected with Theileria equi in in vitro culture using microaerophilous stationary
phase (MASP) technique. Malondialdehyde (MDA) is biomarker of oxidative
damage which is indirectly indicative of lipid peroxidation of erythrocytic membrane. The
continuous MASP culture of T. equi was established for evaluating the oxidative damage in
in vitro condition. Theileria equi–infected erythrocytes (iRBC) were collected from in vitro
cultures at different parasitemia (1%–10%). Theileria equi–uninfected erythrocytes (uRBC)
were also collected for control studies. Malondialdehyde concentrations in iRBC and uRBC
were evaluated. A nonsignificant (P < .001) difference in MDA concentration between
uRBC (339.94 0.80 mM/mL) and 1%–10% of iRBC (357.48 0.82 mM/mL) was observed.
After 1% of T. equi parasitemia, a sequential significant (P < .001) increase in MDA levels
was observed coinciding with increasing T. equi parasitemia. Similarly, osmotic fragility of
iRBC also increases with rise in T. equi parasitemia. Percent hemolysis of iRBC increased
from 13.89% to 26.40% at 1% to 10% parasitemia in 0.85% of sodium chloride solution. The
results of this study demonstrated that horse erythrocytes when infected by T. equi in
in vitro condition undergo oxidative damage and osmotic fragility, which increased with
increasing parasitic load and may be a contributing factor in pathogenesis process of this
disease condition.Not Availabl
