Alkassab, Abdulrahim

Castle, Denise

Eckert, Jakob

Pistorius, Jens

Steffan-Dewenter, Ingolf

English

JKI Open Journal Systems (Julius Kühn-Institut)

Young Scientists Meeting 2019, Kleinmachnow, Germany, November 6-8 Castle et al. Combined stressors of insecticides, fungicides and pollen quality on honey bee health  Denise Castle1,2, Abdulrahim Alkassab1, Jakob Eckert1, Ingolf Steffan-Dewenter2 and Jens Pistorius1 1Julius Kühn Institute, Institute for Bee Protection, Braunschweig, Germany 2 University of Würzburg, Department of Animal Ecology and Tropical Biology, Würzburg, Germany E-mail of corresponding author: denise.castle@julius-kuehn.deHoney bee health has been reported to be affected by multiple factors including resource quality, exposure to agrochemi-cals and pressure of pests and pathogens. Currently, concerns are growing about potential interactions between multiple stressors and their impacts on bee health.  Therefore, we performed a semi-field study to investigate the combined im-pacts of different pollen qualities and ex-posure to a mixture of agrochemicals. Twenty-four tents with crops with a gra-dient of different pollen quality for honey bees were realized: Phacelia with high pollen quality, maize with less pollen quality and tents planted with 50% maize and 50% flower mixtures with diverse pollen qualities. Additionally, the variants were sprayed with a tank mixture of the pesticides Mirage 450 EC (Prochloraz) and Biscaya (Thiacloprid). Various param-eters were investigated such as mortality of adults, larvae and pupae, activity of detoxification enzymes, weight and lon-gevity of newly hatched bees.On the other hand, while several studies have reported the synergism between EBI-fungicides and different classes of in-secticides, few studies have focused on the effects on honey bee larvae and their activity of detoxification enzymes. There-fore, we aimed to investigate the mortal-ity of larvae in the laboratory fed with ar-tificial diet spiced with the EBI-fungizide Prochloraz, the neonicotinoid Thiacloprid and the novel butenolide insecticide Flupyradifurone (active substances) in single and combined exposure.  Our preliminary results show potential synergistic effects on larval mortality with different magnitudes compared to reported effects on adults. This indicates a lower susceptibility of larvae to tested plant protection products. Activity of the primary detoxification enzymes P450, gluthation-S-transferase and acetyl-cholinesterase will be analyzed in col-lected larvae from laboratory as well as semi-field studies. Berichte aus dem Julius Kühn-Institut 206 37

Combined stressors of insecticides, fungicides and pollen quality on honey bee health

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Combined stressors of insecticides, fungicides and pollen quality on honey bee health

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