Hausmann, Ludger

Lima da Silva, Aparecido

Maas Steiner, Daniel Ricardo

Modesto, Lenon Romano

Neri de Almeida, Anna Flávia

Nodari, Rubens Onofre

Welter, Leocir José

English

JKI Open Journal Systems (Julius Kühn-Institut)

Modesto et al. Reactive oxygen species (ROS) activity of grapevine berry upon anthracnose attack Lenon Romano Modesto1, Anna Flávia Neri de Almeida1, Daniel Ricardo Maass Steiner3, Rubens Onofre Nodari1, Leocir José Welter1,2, Ludger Hausmann4, Aparecido Lima da Silva1 1Graduate Program on Plant Genetic Resources, Federal University of Santa Catarina – UFSC, Floria-nópolis, SC, Brazil. 2Federal University of Santa Catarina, Campus of Curitibanos, Curitibanos, SC, Brazil. 3Undergraduate in agronomy, Federal University of Santa Catarina – UFSC, Florianópolis, SC, Brazil.  4Julius Kühn-Institut Institute for Grapevine Breeding Geilweilerhof, Siebeldingen, Germany. E-mail of corresponding author: lenon.modesto@julius-kuehn.deAnthracnose an important disease that occurs regularly in in grapevine areas where conditions during the growing sea-son are usually humid and warm with fre-quent rains during spring months. The pathogen, Elsinoe ampelina, infects pri-marily young tissues, such as shoots and leaves, but also attacks fruits. Most grapevine cultivars grown around the world are susceptible to this pathogen. However, some new bred cultivars of a group denominated PIWI (pilzwider-standsfähige Rebsorten) show tolerance to anthracnose attack but the mecha-nism of resistance is not yet clear. Often, the resistance against fungal diseases is linked with ROS (reactive oxygen spe-cies).  The aim of this work was to study ROS production linked with grapevine re-sistance against anthracnose attack of berries. The experiment was designed based on a bifactorial trial (5x2) using five genotypes, ‘Aromera’, ‘Bronner’, ‘Feli-cia’, ‘Helios’ and GF24 and analyzing ber-ries with or without anthracnose symp-toms. Four bunches with symptoms from natural anthracnose infection were col-lected with three replications, at the phe-nological stage of berry formation. Dis-ease severity was evaluated using images and the software Quant, measuring the percentage of disease. ROS enzymatic ac-tivity, superoxide dismutase, catalase, ascorbate peroxide and guaiacol peroxi-dase, were measured using skins peeled off from berries. All data were used for bifactorial variance analyses followed by Tukey´s test to analyze the relation be-tween genotypes and berries with and without symptoms. Based on the disease severity observed, the genotypes were classified into different resistance levels. GF24 and ‘Helios’ were grouped as re-sistant, ‘Aromera’ and ‘Bronner’ as mod-erately resistant and ‘Felicia’ was consid-ered as susceptible. ROS production showed no interactions between the fac-tors genotypes and berries. Corroborat-ing, the berries with and without symp-toms did not show differences for ROS studied. However, resistance and moder-ately resistance genotypes showed higher ROS activity than the susceptible cultivar. The generation of ROS in mito-chondria plays an important role as a sig-nal for HR-induced cell death in plants. These results suggest that the resistance of berries against anthracnose is linked with ROS production. However, the com-plete ROS route need to be studied in more detail. Berichte aus dem Julius Kühn-Institut 206 1112th Young Scientists Meeting 2019, Kleinmachnow, Germany, November 6-8 

Reactive oxygen species (ROS) activity of grapevine berry upon anthracnose attack

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Reactive oxygen species (ROS) activity of grapevine berry upon anthracnose attack

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