Böge, Lars

Ordon, Frank

Pillen, Klaus

Sannemann, Wiebke

Serfling, Albrecht

Sommer, Leonhard

English

JKI Open Journal Systems (Julius Kühn-Institut)

Young Scientists Meeting 2019, Kleinmachnow, Germany, November 6-8 Böge et al. MAGIC-RESIST: Identification and mapping of effective resistance genes to rust diseases and Fusarium head blight in the MAGIC WHEAT population WM-800 Lars Böge1, Leonhard  Sommer2, Wiebke Sannemann2, Albrecht Serfling1, Frank Ordon1 and Klaus Pillen2 1 Julius Kühn Institute, Institute for Resistance Research and Stress Tolerance, 2 Martin Luther University Halle-Wittenberg (MLU)  E-mail of corresponding author: lars.boege@julius-kuehn.deBasis of the MAGIC-RESIST project is the MAGIC WHEAT population WM-800 which is derived from eight elite wheat varieties (Patras, Meister, Linus, JB As-ano, Tobak, Amber, Safari, Julius). The project aims at the identification of effec-tive resistances against leaf rust and stripe rust and Fusarium head blight (FHB), followed by mapping resistance QTLs by genome wide association studies (GWAS). To achieve this, we conduct field tests at two locations in Quedlinburg and Halle over several years using artificial in-oculation. Field test results are supple-mented by hyperspectral image analyses and high-throughput studies of leaf seg-ments of all genotypes after inoculation with differentiating leaf and yellow rust races. In order to analyze the toxin con-tent of field-harvested grains and to cor-relate data with scoring results the Deox-ynivalenol content will be determined by ELISA.  First analyses of the resistance against rusts and FHB showed large differences between the lines in greenhouse and field trials and rust resistant genotypes were detected. Due to the warm and dry weather FHB was difficult to detect in the field. However, it was possible to detect differences in field-harvested grains. Us-ing a potato dextrose agar, Fusarium has been detected in suspicious grains and was confirmed by detecting conidio-spores in the growing mycelia by micros-copy.  Furthermore, morphological data such as plant height, thousand-grain weight, grains per ear and flowering time will be included into the investigations. In the fu-ture, genotypes that combine resistance QTLs with desired morphological proper-ties will be included into the breeding programs of the cooperating wheat breeding companies. 36 Berichte aus dem Julius Kühn-Institut 206

MAGIC-RESIST: Identification and mapping of effective resistance genes to rust diseases and Fusarium head blight in the MAGIC WHEAT population WM-800

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MAGIC-RESIST: Identification and mapping of effective resistance genes to rust diseases and Fusarium head blight in the MAGIC WHEAT population WM-800

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