Bonse, Sabine

Niehl, Annette

Sprotte, Nico

English

JKI Open Journal Systems (Julius Kühn-Institut)

12th Young Scientists Meeting 2019, Kleinmachnow, Germany, November 6-8Sprotte et al. Illuminating SBWMV-host interaction: Subcellular localization of viral proteins during infection  Nico Sprotte, Sabine Bonse and Annette NiehlJulius Kühn Institute, Institute for Epidemiology and Pathogen Diagnostics, Braunschweig E-mail of corresponding author: nico.sprotte@julius-kuehn.deSoil-borne cereal viruses cause substan-tial crop losses and therefore represent an extensive threat for agriculture in Eu-rope, Asia and America. The bipartite Fu-rovirus Soil-borne wheat mosaic virus (SBWMV) infects several crop species like wheat, rye or barley and is transmitted by a soil-borne plasmodiophorid, called Pol-ymyxa graminis. Resistances against Fu-rovirus infection are barely described, in wheat for e.g. the Sbm1 and Sbm2 genes encode for a translocation resistance, which restricts the infection to the plant roots. The infection of the roots or the translocation of the virus into upper plant tissues depends on the viral movement protein (MP) and coat protein –readthrough (CP-RT) protein. In this study, we modified a SBWMV cDNA clone to express MP:GFP and CP-RT:GFP fusion proteins to uncover their subcellular localization and illuminate their specific functions during virus infec-tion. Both genes were fused to GFP and the modified cDNA clones were used as templates for RNA synthesis. In-vitro pro-duced viral RNA was used for infection of different host plants and the fluorescent proteins were localized by confocal laser-scanning microscopy (CLSM).  Further experiments will aim at identify-ing host components, which are involved in the infection process. This knowledge could provide new ideas for the develop-ment of resistance strategies against soil-borne viruses.  48 Berichte aus dem Julius Kühn-Insitut 206

Illuminating SBWMV-host interaction: Subcellular localization of viral proteins during infection

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Illuminating SBWMV-host interaction: Subcellular localization of viral proteins during infection

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