To identify the level of nurses’ knowledgeand practices regarding the prevention of Cesarean Section (CS) Surgical Site Infection (SSI).Methods: Participants were nurses and midwives working in the labor rooms and postnatal wards. Data collection was conducted at ten government hospitals selected in West Sumatra Indonesia using self-report questionnaires. A total of 201 nurses completed the questionnaires. The levels of knowledge and the levels of practice were categorized as very low, low, moderate, high, and very high.Results: Nurses had low levels of knowledge and high levels of practice regarding the prevention of CS-SSI in Indonesia.Conclusions: Nurses' knowledge and practice in certain areas of the prevention of CS-SSI need to be improved. Education and training programs should be conducted in Indonesia to improve nurses’ knowledge and practice regarding CS-SSI prevention using evidence-based practice