Adema, Coen

English

University of New Mexico

  
Biomphalaria glabrata field isolate (BB02) collected for construction of
BAC library
Biomphalaria glabrata snails were collected by Omar dos Santos Carvalho in Brazil, South America. This
field isolate will be used to generate a strain designated as BB02 (Biomphalaria from Barreiro caught in
2002).
 The genomic DNA from this strain will be the template for production of the BAC library. 
Type location of the BB02 strain of Biomphalaria glabrata
Biomphalaria glabrata snails were collected in the south east of Brazil (19oS59min/44oW02min), from a
stream into a ranch where flowers are grown, in the metropolitan region of Belo Horizonte, district of Barreiro.
The photographs show the collection site and a close-up of the stream.
Contact information Omar dos Santos Carvalho, 
Chefe do Lab. de Helmintoses  Intestinais  
Centro de Pesquisas René Rachou /Fiocruz
Av. Augusto de Lima,  1.715 
30190.002 Belo Horizonte, MG, Brasil
Telephone (+55) 31 3295-3566 Fax 3295-3115
This field isolate is used to generate a strain designated as BB02 (Biomphalaria from Barreiro caught in
2002).  The F1 of the wild caught snails was tested for susceptibility with 3 different Schistosoma mansoni
strains (LE, SJ and AL, maintained at the Section of Mollusks Rearing at  Centro de Pesquisas René Rachou in
Fiocruz, Belo Horizonte Brasil) using a ratio of 10 miracidia/snail. Parasite-susceptible snails from the pre-
existing BB01 strain of Biomphalaria glabrata (maintained over 10 years in the same lab) was used as an
infectivity control.
Outcome of experimental infections, kindly contributed by Omar dos Santos Carvalho :
LE strain of Schistosoma mansoni
     50  Biomphalaria glabrata (BB02) were exposed individually to 10 miracidia.
     2 Snails died and 43 were infected. The percentage of infection was: 89,6%.
  
     50 Biomphalaria glabrata (control BB01) were exposed individually to 10 miracida.
     9 Snails died and 38 were infected. The percentage of infection was: 92,7%.
SJ strain of Schistosoma mansoni were:
     50 Biomphalaria glabrata (BB02) were exposed individually to 10 miracidia.
     6 died and 40 were infected. The percentage of infection was: 90,0%
The BB02 strain was also transported (by means of egg masses) to the Department of Biology, University of
New Mexico, Albuquerque NM, USA. Molecular markers will be used to place this strain among previously
characterized Biomphalaria glabrata isolates. Juvenile BB02 snails will be exposed to the NMRI strain of
Schistosoma mansoni. Other labs may additionally test susceptibility of the BB02 strain to Schistosoma
mansoni also.
Morphological data and sequence information confirm that the BB02 isolate is Biomphalaria glabrata.
   
Molecular characterization of BB02 strain as Biomphalaria glabrata through PCR-RFLP on polyacrylamide
gel, using DdeI enzyme and ITS region
kindly contributed by Omar dos Santos Carvalho :
 Legend
 Lane  1       :  Biomphalaria glabrata from Jacobina
                                                    (State of Bahia, Brazil). 
 Lane  2 -11 :  Biomphalaria glabrata (BB02). 
 Lane 12       :  Biomphalaria glabrata from Esteio
                                                    (State of Rio Grande do Sul, Brazil).
 
  
 Partial sequences of the 16S rDNA and NADH dehydrogenase 1 genes from BB02 were characterized and
deposited in GenBank under accession numbers AY737280 and AY737281, respectively.
 
  


\u3ci\u3eBiomphalaria glabrata\u3c/i\u3e field isolate (BB02) collected for construction of BAC library

https://digitalrepository.unm.edu/cgi/viewcontent.cgi?article=1003&amp;context=biomphalaria-genome

\u3ci\u3eBiomphalaria glabrata\u3c/i\u3e field isolate (BB02) collected for construction of BAC library

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University of New Mexico