Effect of metatopolin on micropropagation and rooting apple type Gala

Abstract

Zbog nuspojava benzilaminopurina (BAP-a) kao što su nekroza vrha izdanka, inhibicija zakorjenjivanja te teškoće pri aklimatizaciji u literaturi se kao alternativa BAP-u navodi hidroksilirani analog BAP-a – metaTopolin (mT). Kroz ovaj rad istraživao se utjecaj metaTopolina i BAP-a na mikropropagaciju jabuke sorte Gala pri čemu se procjenjivala duljina i razvijenost izdanaka te količina klorofila; duljina, širina i površina lista te broj puči po površini lista. Izdanci su zakorjenjeni na MS mediju s dodatkom 2 mg/l IAA i procijenjena je uspješnost zakorjenjivanja s obzirom na prethodni medij (prethodno korišten citokinin). Izdanci razvijeni na podlozi sa citokininom mT imali su veću lisnu površinu, duljinu i širinu listova. Sadržaj klorofila u listu i broj puči po površini lista se nisu statistički razlikovali za biljke sa oba tretmana dok je zakorjenjivanje bilo uspješnije kod biljaka sa tretmana BAP-om.Due to the side effects of benzylaminopurine (BAP), such as shoot top necrosis, inhibition of rooting, and difficulty in acclimatization, a hydroxylated analogue of BAP - metaTopolin (mT) has been cited as an alternative to BAP. Through this work, the influence of metaTopolin and BAP on micropropagation of Gala apples was investigated, estimating the length and development of shoots and the amount of chlorophyll; the length, width and area of the leaf and stomatal density. The shoots were rooted on MS medium supplemented with 2 mg / l IAA and the rooting performance was evaluated with respect to the previous medium (cytokinin previously used). The shoots developed on cytokinin mT substrate had larger leaf area, leaf length and width. Chlorophyll content in the leaf and stomatal density were not statistically different for plants with both treatments, whereas rooting was more successful with plants with BAP treatment