Assis, Helena C.S.

Carlos, Rose Maria

dos Santos Silva, Eldevan

Guiloski, Izonete C.

Skibsted, Leif Horsfelt

Souza, Leonardo de C. e

Vital, Maria A.B.F.

English

Copenhagen University Research Information System

u n i ve r s i t y  o f  co pe n h ag e n  Københavns UniversitetMagnesium metallic complex coordinated phenolic used for inhibitor of reactiveoxygen species (ROS) in biological systemdos Santos Silva, Eldevan; Carlos, Rose Maria; Assis, Helena C.S.; Guiloski, Izonete C.;Vital, Maria A.B.F.; Souza, Leonardo de C. e; Skibsted, Leif HorsfeltPublication date:2016Document versionPublisher's PDF, also known as Version of recordCitation for published version (APA):dos Santos Silva, E., Carlos, R. M., Assis, H. C. S., Guiloski, I. C., Vital, M. A. B. F., Souza, L. D. C. E., &Skibsted, L. H. (2016). Magnesium metallic complex coordinated phenolic used for inhibitor of reactive oxygenspecies (ROS) in biological system. Abstract from 13th European Biological Inorganic Chemistry Conference,Budapest, Hungary.Download date: 03. Feb. 2020 Magnesium Metallic complex coordinated phenolic used for inhibitor of Reactive oxygen species (ROS) in biological system Eldevan dos Santos Silva1*, Rose Maria Carlos2,   Helena C. S. Assis3, Izonete C. Guiloski3, Maria A. B. F. Vital3, Leonardo de C. e Souza3  and Leif H. Skibsted1 1 - Food Chemistry Rolighedsvej 30 1958 Frb. C - +4535333221 - University of Copenhagen – Dannmark 2 –Departament of Chemistry University Federal of São Carlos- Rodovia Washington Luís, km 235-SP- São Carlos SP- CEP - 13565-905- Brazil - Telefone:+55163351-8636 3 – Farmacologia -Centro Politécnico- University Federal of Paraná - Jardim das Américas CEP 81531-980 - Curitiba, PR, Brazil  * eldevansilva@hotmail.com  Mitochondrias are essential organelles for the life and death of the cell, participating of the cellular energy metabolism as well as in the control of programmed cell death1. But mitochondria are responsible for the major source of reactive oxygen species in vivo, thus being mitochondrial oxidative damage one of the main causes of many chronic diseases. This work will show the results about the potential of new antioxidant compounds based on the complex Mg-isoflavones which can be used to inhibit reactive oxygen species (ROS) in vivo. Phenolic acids have important roles in the biological system, highlighting the prevention and treatment of noncommunicable neurodegenerative and chronic diseases. The use of complex Mg-isoflavones showed an IC50 of 96.00 ± 4.12 M to inhibit the stable radical DPPH shown in Figure 1 A.   A)   Mg-isoflavones – DPPH – IC50= 94.70 ± 4,12 3330 3340 3350 3360 3370 3380 3390 3400 3410300MMagnetic field (G)Control - DPPHIntensity37M B) In vivo studies - Wistar rats    Figure 1: Inhibition of DPPH radical complex Mg-isoflavones using Electron paramagnetic resonance (EPR) (A),  Biochemical studies (in vivo) in the brain and kidneys of Wistar rat (B).   Biochemical studies in rats were performed by using the complex Mg-isoflavones on enzymes superoxide dismutase (SOD) and glutathione (GSH). It was shown an increased SOD activity in the hippocampus, thu indicating that this complex can stimulate the production of SOD in the brain and stimulate the production of glutathione (GSH) in kidney. This result is of great importance as GSH is the best antioxidant in the human body. Moreover, it is not possible to verify that Mg-isoflavones can induce toxicity to animals at the tested concentration (3 mg/mL maximum solubility in saline solution 5%, w/v). Acknowledgements: São Paulo Research Foundation – FAPESP the scholarship proce-2015/23146 1.  Brand, M. D.; Affourtit, C. Esteves TC, Green K, Lambert A. J.; Miwa, S.; Pakay, J. L.; Parker, N. Free Radic Biol Me. 2004, 37, 755–767 2. Halliwell, B.; Aeschbach, R.; Loliger, J.; Arouma, O. I. Food Chem.Toxicol. 1995,v. 33, 601 

Magnesium metallic complex coordinated phenolic used for inhibitor of reactive oxygen species (ROS) in biological system

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Magnesium metallic complex coordinated phenolic used for inhibitor of reactive oxygen species (ROS) in biological system

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