Mabagala, R.B.

Mbega, E.R.

Wulff, Ednar Gadelha

English

Copenhagen University Research Information System

u n i ve r s i t y  o f  co pe n h ag e n  Københavns UniversitetPlant extracts and essential oils in seed treatment:Mbega, E.R.; Mabagala, R.B.; Wulff, Ednar Gadelha; Mortensen, Carmen NievesPublication date:2011Document versionPublisher's PDF, also known as Version of recordCitation for published version (APA):Mbega, E. R., Mabagala, R. B., Wulff, E. G., & Mortensen, C. N. (Ed.) (2011). Plant extracts and essential oils inseed treatment: production of healthy tomato transplants. Danish Seed Health Centre for Developing Countries,University of Copenhagen.Download date: 02. Feb. 2020       Production of healthy Tomato transplantsDenmark:Danish Seed Health Centre (DSHC)                         Faculty of Life Sciences, University of  Copenhagen Højbakkegård Allé 9, 2630 Tåstrup, Denmark      Phone: +45 35 3333186; E-mail: cnm@life.ku.dk;   www.dshc.life.ku.dkTanzania:African Seed Health Centre  (ASHC)           Sokoine University of Agriculture                        P.O. Box 3005, Morogoro, Tanzania Phone: +255 232 600 573; Fax: +255 232 600 574 E-mail: rbm@afshc.or.tz; www.afshc.or.tzCompiled by:E. R. Mbega, R.B. Mabagala (AfSHC)E.G. Wulﬀ , C.N. Mortensen  (DSHC)Th e leaﬂ et was compiled from                     outputs of the  Danida Enreca project Life-731 at the time of writing.  Plant extracts and essential oils in seed treatmentResearch outputs Enreca Project LIFE-731                                                                                                                                                  «Application of Plant Extracts and Essential Oils  as Seed Bactericides»    Could be advantageous to small scale  farmers in the production of healthy      and robust tomato transplants:                Easily available      Environmentally friendly                       Biodegradable                     Low toxicity to animals                       No phytotoxic effects            (germination, vigour, plants      weight)                                                                                                           « Best Plant Extracts »                                                                                                          Aloe vera (Aloe), Coffea arabica (Processed coffee),  Glabra urelensis (Liquorice), Yucca schidigera (Yucca)          « Best Plant Essential Oils »                                                                                                             Eucalyptus globules (Eucalyptus), Rosmarinus of-ﬁ cinalis  (Rosemary) and Melaleuca viridiﬂ ora (Niaouli) at 2% (v/v) inhibited the growth of Xan-thomonas and  were the most eﬃ  cient  in control-ling BLSPreparation of plant extracts:  Boil 2 g of air-dry chopped plant parts  (bark, leaves, roots, f lowers, and seeds) in 20 ml of sterile dis-tilled water Filter the plant extract suspensions using two-layered cheese-cloth  Adjust  volume to 20ml with water  Keep plant extracts in 30ml clean tubes with  screw caps Autoclave plant  extracts at 121 °C  for 15 min and kept at 4 °C until useBioa ss ays Bacterial leaf spot (BLS)A disease caused by diﬀ erent seed-borne pathogenic xanthomonads: Xanthomonas eu-vesicatoria, X.vesicatoria, X. perforans and X. gardneri is a serious constraint of tomato (Ly-copersicum esculentum Mill) production causing signiﬁ cant losses in yield and quality. Th e eﬃ  cacy of aqueous extracts obtained from 84 plant species and 11 esential oils to control BLS was evaluated with infected tomato seed under laboratory and greenhouse conditions. Tomato seeds of the cv. Tanya from Tanzania served as a model for the studies. Seed samples infected with a strain of X. perforans NCPPB 4321 were soaked overnight in 10% (v/v) aque-ous plant extracts and essential oils at 2% (v/v) concentration.Plate 50μl of seed extract or oil suspension onto agar substrate by steaking: incu-bate at 28 °C for 1-2 days                                                                                                                                                                              BLS colonies on mTB agar indicate: no inhibi-tion of the pathogen by             the seed treatment BLS symptoms on tomato leaves and fruits Production of healthy tomato transplants in the greenhouse Seed treatments:Prepare water suspensions of plant extracts (10%) or essential oils (2%)                                                                                               Incubate overnight at room temperature and if possible under shakingAir-dry the seeds onto      absorbent paper and keep seed refrigerated until use                                                                                                         Evaluation of eﬃ  cay of seed treatments             Plant seeds in soil (14-21d) under moist conditions at 28 °C: higher number of healthy transplants from best seed treatments with 75 -100% reductions of BLS disease incidence                                                               No eﬃ  cay of seed treat-ment:  tomato leaves with  Add 20seeds/ml of        treatment                   In planta tests:                              No eﬃ  cay of seed treatment (left and right: high incidence of BLS); good control of BLS Eﬃ  cacy of seed treat-ment:  low number of BLS symptoms   In vitro tests:

Plant extracts and essential oils in seed treatment::production of healthy tomato transplants

https://curis.ku.dk/ws/files/37990190/seed_treatment_leaflet_27.4.11.pdf

Plant extracts and essential oils in seed treatment::production of healthy tomato transplants

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