In this study, native polyacrylamide gel electrophoresis (Native-PAGE) and sodium dodecyl
sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were applied to the serum proteins of
Leuciscus cephalus, Acanthobrama marmid and Chondrostoma regium (Cyprinidae) fish taken
from) the Karakaya Dam (Malatya, Turkey). The electrophoregrams showed that there were
similarities and differences in the molecular weight (MW) of the serum proteins among the
three species. In the Native-PAGE, the total number of serum protein bands of L. cephalus, A.
marmid and C. regium were 5, 8, 7, respectively. In the SDS-PAGE, 13 protein bands were
obtained in L. cephalus, 11 in A. marmid and 11 in C. regium. In the electrophoregrams
obtained from native-PAGE, the MW of the protein bands were found to be different in the
fish species except the 80.3 kD protein band which was detected in the three fish species. In
the SDS-PAGE, the protein band of 21.7 kD was observed in the three fish species. Protein
bands of 63.4, 52.3 and 49.5 kD were present only in L. cephalus and C. regium. On the other
hand, it was seen that other protein bands had similar molecular weights