Abstracts from the April 12-14, 2019 MASC Conferenc

Hughes, Karen W.

Kingsley, Nathaniel H.

English

University of Tennessee, Knoxville: Trace

University of Tennessee, KnoxvilleTrace: Tennessee Research and CreativeExchangeMiddle Atlantic States Mycological Conference2019 Conferences at UT8-2019After the fire: biodiversity of fungal endophytes ingerminating Table Mountain Pine seedlings (Pinuspungens)Nathaniel H. KingsleyUniversity of Tennessee, KnoxvilleKaren W. HughesUniversity of Tennessee, KnoxvilleFollow this and additional works at: https://trace.tennessee.edu/masmcThis Presentation is brought to you for free and open access by the Conferences at UT at Trace: Tennessee Research and Creative Exchange. It has beenaccepted for inclusion in Middle Atlantic States Mycological Conference 2019 by an authorized administrator of Trace: Tennessee Research andCreative Exchange. For more information, please contact trace@utk.edu.Recommended CitationKingsley, Nathaniel H. and Hughes, Karen W., "After the fire: biodiversity of fungal endophytes in germinating Table Mountain Pineseedlings (Pinus pungens)" (2019). Middle Atlantic States Mycological Conference 2019.https://trace.tennessee.edu/masmc/13Mid-Atlantic States Mycological Conference (MASMC) University of Tennessee – Knoxville 12-14 April 2019  ABSTRACTS - Oral Presentations   After the fire: biodiversity of fungal endophytes in germinating Table Mountain Pine seedlings (Pinus pungens) Nathaniel H. Kingsley, Karen W. Hughes Department of Ecology and Evolutionary Biology, University of Tennessee  Following the November 2016 wildfires in the GSMNP, Table Mountain Pine (Pinus pungens) seedlings were observed germinating in three high burn areas (Cove Mt., Baskins Creek, and Two Mile Lead).  Needles were sampled from both the bottom and tops of pine saplings.  Needles were sterilized using sterilization techniques from U’Ren et al. then cultured on malt extract-agar (MEA) medium. Fungal endophytes growing from the needles were subcultured to clean MEA plates.  DNAs were extracted from cultures and the internal transcribed spacer (ITS) region was sequenced. In all, 200 endophyte cultures were isolated and sequenced (Cove Mt., 89; Baskins Creek, 54; Two Mile Lead, 57) producing 58 unique genotypes (Cove Mt., 34; Baskins Creek, 26; Two Mile Lead, 20). Following the general trend of endophyte inoculation, the needles at the bottom had a higher culture success rate and overall diversity when compared to those sampled from the tops of the saplings. The most abundant taxa recovered were Alternaria, Cladosporium, Diplogelasinospora, Epiccocum, Xylaria, and Sphaerosporella. Only 6 genotypes were found across all three sites, with an additional nine genotypes shared across two of the three sites (Cove Mt. + Baskins Creek, Cove Mt. + Two Mile Lead). The high biodiversity across all three sites demonstrates the ability of pine to serve as a host for a broad scope of fungal endophytes.   

After the fire: biodiversity of fungal endophytes in germinating Table Mountain Pine seedlings (Pinus pungens)

https://trace.tennessee.edu/cgi/viewcontent.cgi?article=1012&amp;context=masmc

After the fire: biodiversity of fungal endophytes in germinating Table Mountain Pine seedlings (Pinus pungens)

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University of Tennessee, Knoxville: Trace