The aim of the study was to find out the potential prognostic value of proliferation
activity and apoptosis in cholesteatoma and granulation tissue removed
during middle ear reoperation in recurrent middle ear inflammation.
Granulation tissues and recurrent cholesteatoma were analysed after being surgically
removed from the middle ear in a group of 25 patients qualified for
middle ear reoperation procedure. Paraffin sections were stained with haematoxylin
and eosin according to Mallory’s method. Immunohistochemical reaction
Anti-PCNA was performed. Apoptosis was evaluated using the TUNEL method.
The percentage of PCNA-positive cells was 42–95% in the matrix of the
cholesteatoma and 29–81% in the perimatrix. In the granulation tissue it was
35–75%. The percentage of apoptotic cells was 12–73% in the matrix of the
cholesteatoma, 5–72% in the perimatrix and 1–65 % in the granulation tissue.
The prognostic value of the parameters studied in the recurrent middle ear inflammatory
process is questionable, probably due to the small number of cases
under examination
