Immunohistochemical characteristics of neurones innervating the porcine uterus
located in paracervical ganglia were studied with a combination of retrograde
fluorescent tracing and immunofluorescence. Retrograde fluorescent tracer Fast
Blue (FB) was injected into the uterine horn and uterine cervix. The presence
of biologically active substances, tyrosine hydroxylase (TH), neuropeptide Y (NPY),
vasoactive intestinal polypeptide (VIP), galanin (GAL), Met-enkephalin-Arg-Gly-Leu
(MEAGL) and calcitonin gene-related peptide (CGRP) was studied in FBpositive neurones
localised in paracervical ganglia. FB-positive neurones containing TH, NPY, VIP
and MEAGL were numerous, while those containing CGRP were scarce. The results
pointed to some species-related differences in immunohistochemical coding of neurones
of paracervical ganglion responsible for uterus innervation
