The in vitro viability and growth of fibroblasts cultured in the presence of different bone grafting materials (NanoBone® and Straumann Bone Ceramic®)
Different clinical applications, including dentistry, are making increasing demands
on bone grafting material. In the present study we have analysed the viability,
proliferation and growth characteristics of fibroblasts cultured in vitro together
with two different bone grafting materials, NanoBone® and Straumann Bone
Ceramic®, over a period of 24 and 28 days respectively. Viability was measured
at least every 72 hours by using the alamarBlue assay, a test that measures
quantitatively cell proliferation and viability but does not require cell fixation or
extraction. After one week of culture fibroblast viability was as high as in controls
for both grafting materials and remained high (> 90%) for the duration of
the experiment. Cell growth was evaluated microscopically. Scanning electron
microscopy revealed a dense fibroblast growth at the surface of both bone grafting
materials after three weeks of in vitro culture. Generally, our in vitro analyses
contribute to further insights into cell - scaffold interactions