A balloon-borne instrument has been used to detect cosmic-ray antiprotons. These are identified topologically by the appearance of annihilation prongs in a thick lead-plate spark chamber. The initial recording of the data is enriched in potential antimatter events by a selective trigger. After a small subtraction for background, 14 identified antiprotons yield a flux of 1.7 plus or minus 0.00005 antiproton/(sq m ster sec MeV) between 130 and 320 MeV at the top of the atmosphere. When combined with higher energy antiproton flux measurements, this result indicates that the antiprotons have a spectrum whose shape is the same as that of the protons, but with a magnitude reduced by a factor of 1/3000
