Determination of phospholipids extracted from Tsukamurella paurometabola C-924 after freeze-drying and during the subsequent storage

Abstract

The total lipids of Tsukamurella paurometabola C-924 were analyzed after freeze-drying. Seven individual lipids classes were identifiednamelyneutrallipids(NLs),fattyacids(FAs),phospholipids(PLs),sterolester(SEs),triglycerides(TGs),diglycerides (DGs) and monoglycerides (MGs). The principal fatty acids identified in most lipid classes were palmitic (C 16:0), palmitoleic (C 16:1), oleic (C 18:1), linoleic (C 18:2), and linolenic (C 18:3). PLs were the major constituents and accounted for 50-60% of the total lipids. PLs were fractionated. PLs of Tsukamurella paurometabola content phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), sphingomyelin (SM), lysophosphatidylcholine (LPC) and phosphatidylglycerol (PG). It was observed that PG had the highest proportion at most points relative to other PLs and was the predominant component of PLs (30%-56%). Evolution of individual rate was followed during storage at 20°C and 40°C with or without lithothamne400®, respectively