Galineau, Jeremy

Morrin, Aoife

DCU Online Research Access Service

EMµ: The next generation of separation scienceJeremy Galineau HPLC 200929/06/2009Outline• Introduction to monolithic columns• Monolithic µchips• EMµ:• micro-structured monolith• electro-polymarization• Preliminary results• ConclusionAdvantages ocolumns• Laminar flow• Less back pressure:• higher flow rate• faster separations• Decrease A-term and CDeemter equationf monolithicPorous polymer monolith used in separation column-term of the Van From Chemical & engineering news, vol 87, 20, p. 11-18Limitations ocolumns• Lack of reproducibility:• individual preparation• chemical bonding (ex: C18 on silica monolith)PREVENTING WIDESPROF MONOLITHIC COLUMNSf monolithic EAD ACCEPTANCE Monolithic µchipsReproducibility issuesHow can repissues be oroducibilityvercome ?Electroactiveµchips(EMµ) monolithiEMµ(electroactive monMµ overcomes repr• Micro-structuring the monolithic stationary phaseby:• Electro-polymerispolymerolithic microchip)oducibility issuesing the monolithicMicro-structured monolith• Periodic structure provides:• reproducibility• further decrease of A• large flow-through pores• small skeleton size-termElectro-polySeparation channel integrated into a 3precise control over the polymerisation potentialelectrochemical cell:formation of a highly reproducible polymermerisation-electrode Advantages of astationary phaseAllows precise tuning of stationary phase before AND during separations, controlling:• Retention factors• Hydrophobicity/ hydrophilicity• Pore size• Ionic capacityn electroactivs separation on polymer possible?• Electroactive polymer polymglassy carbonSeparation of p- and m-toluic acid (left)phthalates (right) on PP/SDS/RVC: mobile55/45, H,O/CH,OH, 1 mL/min;an electroactiverized onto «crushed» How is the EMµ mo• 3 step process:1. Formation of polystyrene bead template:• colloidal crystallization using capillary forces 2. Electro-polymerization of polymer through the templatenolith fabricated?EMµ(electroactive monolithic microchip)EMµ(electroactive monolithic microchip)EMµ(electroactive monolithic microchPrelimenary resultsTemplatePrelimenary resultsTemplatePrelimenary resultsStructured monolithPrelimenary resultsStructured monolithConclusionControled and reproducible growth of microstructured monolith:TemplatingElectro-polymerizationNext step:Do separations on the monolith to test chromatographic propereproducibility.-rties, versatility and AcknoledgementsProf. Malcolm Smyth Dr. Blanaid White Dr. Aoife MorrinThank you

EMµ: the next generation of separation science.

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EMµ: the next generation of separation science.

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