Although facultative anaerobe Sporolactobacillus inulinus lacks electron transport chain, this genus grow well under aerobic condition the same as anaerobic culture and final metabolic product on aerobic condition is only acetic acid instead of lactic acid of anaerobic condition. Therefore related enzymes to pyruvate metabolism (lactate dehydrogenase, pyruvate dehydrogenase complex, pyruvate oxidase and pyruvate decarboxylase) and closely enzymes on metabolism of acetylCoA (phosphotrans acetylase, acetate kinase, acetyl-CoA hydrolase, acetaldehyde dehydrogenase, and alcohol dehydrogenase) were measured enzymatic activities respectively. The catabolism from pyruvate to acetic acid under aerobic condition took place by pyruvate dehydrogenase complex, acetyl-CoA phosphotrans acetylase and acetyl kinase. NADH produced from EMP pathway and acetyl-CoA reoxidized by NADH oxidase and H_2O_2 from NADH oxidation scavenged with NADH peroxidase of S. inulinus. So we proposed metabolic pathway of glucose to acetate under aerobic condition of S. inulinus