The purpose of this research is to shed light on the involvement of the Vav2 guanine nucleotide exchange factor in the Xenopus laevis EphA4 signaling pathway. In the first phase of the project, the presence of Vav2 mRNA within seven different Xenopus laevis embryogenic stages was detected via embryogenic stage collection, retrotranscription, PCR, using forward and reverse Vav2 primers, agarose gel electrophoresis, and UV gel visualization. In the second phase of the project, Western Blotting techniques were used to determine the presence of Vav2 protein in Xenopus laevis embryogenic stages
