Aim: The aim of the study is to determine the frequency of aspiration, nebulization and oxygenization practices and factors that affect these practices after tracheostomy operation in seven days period.Method: This cross-sectional study was carried out in internal intensive care unit in a university hospital in Turkey. The sample of the study consisted of 60 patients with tracheostomy. Data was collected by using two forms. First one was patient descriptive characteristics form and second one was patient observation form about aspiration, nebulization and oxygenization practices.Results. Mean of aspiration number was 9.9±1.3, and aspiration was carried out more frequently than oxygeniation (8.4±1.7) and nebulization (6.5±1.5) practices. Patients’ age, their habit of cigarette smoking and having chronic disease situations affect aspiration, nebulization and oxygenization practices patients with tracheostomy (p<0.001).Conclusions. The frequency of aspiration, nebulization and oxygenization practices was changed according to post-operation days and affected by some of patients’ descriptive characteristics