The aim of this study is to determine whether there is a significant difference in Islamic moral values of pre-service teachers in secondary schools according to their gender, educational departments, universities, parents’ educational level, parents’ economical level and region. Survey model is carried out in this study. The participants of the study are composed of 554 pre-service teachers from Sakarya University and Aegean University. The data were collected by a five-point Likert scale Islamic Moral Values and analyzed with SPSS. As a result of the study, it is seen that there is a significant difference between the Islamic moral values of pre-service teachers according to their educational departments, universities, parents’ educational level, parents’ economical level and region. On the other hand, there isn’t a significant difference between the Islamic moral values of pre-service teachers according to their gender