Solid-phase extraction (SPE) procedures are being used increasingly in all types of drug testing laboratories, including those, which carry out forensic toxicological analysis. Extraction techniques are used to isolate chemical substances from biological and post-mortem substrates in order to be identified by chromatographic methods. The advantages of such procedures include decreased operation time, reduced solvent volumes and increased extraction efficiency.The present work describes a novel and faster SPE technique, without column preconditioning, for the extraction of amphetamine from spiked biological and post-mortem specimens. Abselut NEXUS columns were used for the extraction from spiked blood, bile, vitreous humour, pericardial fluid, cerebrospinal fluid, liver, brain, bone and bone marrow.The analysis of amphetamine was accomplished by gas chromatography after derivatisation with heptafluorobutyric anhydride (HFBA). The recoveries of amphetamine ranged from 78.5% (pericardial fluid) to 87.5% (bile), with relative standard deviations less than 8.0
