Roth, Olivia

Tanger, Isabel

Wagner, Kim-Sara

OceanRep

• The microbiota is significant for the development, digestionand immunity of the host• Originally obtained from the environment, the microbiota isthen vertically transmitted from parents to offspring• Initial microbial translocation boosts the offspring’s immuneresponse• Translocation of microbiota mainly occurs through processesthat are unique to the maternal bodySex-specific parental transfer of microbiotaKim-Sara Wagner*, Isabel Tanger & Olivia RothGEOMAR Helmholtz Centre for Ocean Research Kiel, Marine Evolutionary Ecology,Düsternbrooker Weg 20, 24105 Kiel, GermanyBoosting the immune responseMore information about our work:When fathers are pregnant*Corresponding authorkwagner@geomar.de[malePREG]Phase I ­ Current research: In vitroPhase II - Outlook: In vivoPregnancyBirthBreastfeedingBoostRegulationState of the art: microbiota composition of the sex-role reversedpipefish Syngnathus typhle differs between female gonads andmale brood pouch (Beemelmanns et al., 2019).Research aims:1. Assess the initial microbial colonization of S. typhle juveniles2. Determine the role of sex-specific microbiota in immunetransfer3. Evaluate the maternal and paternal contribution to immunetransfer1. Isolation of sex-specific microbiotaExperimental Design2. Cultivation and purification of bacterial colonies3. 16S rRNA Genotyping4. Antibiotic Resistance Testing7. Assessment of F1 microbiota composition and immune response6. Sex-specific microbiota exchange and GFP labeling to track vertical transmission5. Application of antibiotics to deplete natural microbiotaSex and pregnancy united  Female pregnancy systems Pipefish Syngnathus typhleSex and pregnancy separated  Eggs EggsPregnancy Pregnancy

Sex-specific parental transfer of microbiota

http://oceanrep.geomar.de/48344/1/Poster_GRC_YIRD_Wagner.pdf

Sex-specific parental transfer of microbiota

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