5’-Deoxy-5’-hydrazinylguanosine as an initiator of T7 RNA polymerase-catalyzed transcriptions for the preparation of labeling-ready RNAs

Abstract

5′-deoxy-5′-hydrazinylguanosine was incorporated into the 5′-termini of RNA transcripts using T7 RNA polymerase. Transcriptions provided 5′-hydrazinyl-RNA that was readily labeled and purified. The use of fluorophore-labeled material was validated in an endoribonuclease activity assay